FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT47.017543859649122Clontech SMART CDS Primer II A (100% over 26bp)
CAGCAAAAGTCTAAAAAGTTACAAACTGGCAACCCATTGTCATGCCTCAA23.508771929824561No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG23.508771929824561No Hit
ATTCCATTCCTTTCCATTAGAAGATGATTCCATTCGAGACCATACGATGA23.508771929824561No Hit
CATCAGCAGCGCGAACGCGGAACTCACGGCTCGCTTTGGCGGCAATAAAT23.508771929824561No Hit
GCAACATGCAATGTACTCATATAAGAAGCCTACATATATACCCTGAACCT23.508771929824561No Hit
GCCCATGCCTGCCCGAAGGTCTCGGCCATCACGGCCACGCCGGGCGGCGT23.508771929824561No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23.508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG23.508771929824561No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG23.508771929824561No Hit
GTTCTCTAAAATGCAAGCATAGAGCCGTGCAAGTACTATTACACACGGGA11.7543859649122806No Hit
GGGCGAGTATCCGGTGATTCCGCCGGTGCGCCAGGGCGCCATGGCGCACT11.7543859649122806No Hit
TTCTCTGTCTCTCTTTGGATGGTGTCTTTCATTCACCATTGCAAGAAGTA11.7543859649122806No Hit
GAGGATATATATTTTATCATCAATTGCTAAATATATATGACAGTAATCTC11.7543859649122806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
CCTGCGTGCGCGGGCCGACGAGATCGCCATGCTGCTCACCGCCGAGCAGG11.7543859649122806No Hit
GGAAAGGAAATATATGCTTCAGCCTCCCATCAGCAGCAGACTCTGAGCTG11.7543859649122806No Hit
GCCCTGGACGACACGTCAATAGGGGAGAAGAAGACCTGTCTCTTATACAC11.7543859649122806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTT11.7543859649122806No Hit
TCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAA11.7543859649122806No Hit
TCTTATAGTGTCTACTCATTGGTCTTTGTTCTGCCCAGTGATAACAATGG11.7543859649122806No Hit
GTGTAATCTACTGAAAAATATACTGAAATTTCACTTGTAATTGCAATCTC11.7543859649122806No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT11.7543859649122806No Hit
GTCTTGAACTCCTGACCTCAGGTGATCCATCTGCCTCGGCCTCCCAAAGT11.7543859649122806No Hit
GTACTAGAAGAGTTTTTGTGGGGCTGTTGTTAATTATTTCTTTTTTTTTT11.7543859649122806No Hit
GGCATATTCTTAGAAGGAATCTCTGATTTGAATCATGTCATACTTAAACA11.7543859649122806No Hit
GTTTATGTCTACCATGTGTCAACATTCTGGACCCTGGGAACTTTCTGGCT11.7543859649122806No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC11.7543859649122806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAGTACCT11.7543859649122806Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAACAATGAT11.7543859649122806No Hit
TCTTATAGTGTCTACTCCTTGGTCTTTGTTCTGCCCAGTGATAACAATGG11.7543859649122806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACAAATGCTT11.7543859649122806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.7543859649122806No Hit
CTGTAATATATTTTGAAGTCAAGTAACACGAGCTCTCCTGCTTTGTTCTT11.7543859649122806No Hit
GATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGC11.7543859649122806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACGG11.7543859649122806No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATAC11.7543859649122806Clontech Universal Primer Mix Long (96% over 28bp)
ATATCACATTCTTTCTTGTGGACCACCAAATTGAAGGCTTTCTTGTAATT11.7543859649122806No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.7543859649122806Clontech SMART CDS Primer II A (100% over 26bp)
CTGATGATCTGTCCTGCCCGCAGCGCCAGCTGCGGGCAGGACAGATCATC11.7543859649122806No Hit
ACATCTCTATGCACATAAACTAGAAAATCTAGAGAAAATAGATAAATCCT11.7543859649122806No Hit
GATCAAGTTGCCTGCAGATCTGGTGTCTGGTGATGCTCCTCTTTTTGAAT11.7543859649122806No Hit
AATCTCCCCAGCGGCTAGCATGTGTAGTGCCTGTCTCTTATACACATCTG11.7543859649122806No Hit
GTACTTTTTTTTATTTTTTTTTTTTTTTTGCGTTGAAACCCACACCATAC11.7543859649122806No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT11.7543859649122806Clontech SMART CDS Primer II A (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)