FastQCFastQC Report
Tue 10 May 2022
EGAF00003636065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063213
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG105890.9959434280807327No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA66600.6264031760334007No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG64170.6035479250159658No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG61700.5803164558747871No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC55080.5180523563951908No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT47080.442808731646434No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT45330.4263491887326434No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC43270.4069739553598385No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC42790.4024593378749131No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG42730.4018950106892975No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC38100.35834776286595443No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA37780.35533801787600416No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG35390.33285898498231303No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG33070.31103833380517354No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT30340.2853614468596603No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG29500.27746086626104083No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA29310.27567383017325786No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG26040.2449179985572035No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC24460.23005738266932402No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT22100.20786051336844075No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT22030.2072021316518891No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC21910.20607347728065778No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA20870.1962918060633194No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA20810.19572747887770373No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT20490.19271773388775346No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC20260.19055447967622668No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG19830.186510134845981No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA19820.18641608031504506No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG19370.18218362642292749No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA19310.1816192992373118No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG19010.17879766330923344No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19000.17870360877829747No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC18660.1755057547264753No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG18330.1724019552055891No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA18210.17127330083435774No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC17890.16826355584440747No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC17790.167323010535048No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG17720.1666646288184964No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC17340.16309055664293046No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC17300.16271433851918665No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG16910.15904621181268477No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC16760.15763539384864556No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT16250.15283861277091232No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA16240.15274455823997637No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA16190.15227428558529665No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA16090.15133374027593718No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA16050.1509575221521934No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA15760.14822994075505097No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG15750.148135886224115No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT15670.14738344997662745No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC15610.14681912279101175No Hit
GCGGTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGA15470.14550235935790853No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC15160.1425866688988942No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC15110.14211639624421446No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG14810.1392947603161361No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC14780.13901259672332825No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC14780.13901259672332825No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG14750.13873043313052041No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT14550.1368493425118015No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTCACCACCCCTTGCTCAGCCT14460.13600285173337798No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT14140.1329931067434277No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC13870.13045363440815716No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG13600.1279141620728866No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC13570.12763199848007878No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA13360.12565685333042392No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT13240.12452819895919255No Hit
AGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT13170.12386981724264094No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC12910.12142439943830634No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA12860.12095412678362662No Hit
TAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTA12810.12048385412894687No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT12710.11954330881958743No Hit
CTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTA12710.11954330881958743No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG12520.11775627273180445No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA12280.11549896398934174No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC12270.11540490945840579No Hit
ATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAG12140.1141822005562385No Hit
TTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTG11950.11239516446845553No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC11670.10976163760224904No Hit
TCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGGGGTGGT11670.10976163760224904No Hit
ACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT11600.10910325588569741No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG11520.10835081963820985No Hit
GATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCAC11430.10750432885978632No Hit
GAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCT11410.10731621979791442No Hit
GCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGA11360.1068459471432347No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT11330.10656378355042688No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT11210.10543512917919552No Hit
GCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC11010.1035540385604766No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC11000.10345998402954064No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC10830.10186105700362956No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA10820.10176700247269363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)