FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGAAGAGCAGCAAGTCGGCCTCCCCGGGCCCAGCTCCGTCCTCTCG23.225806451612903No Hit
TGCCCTTCAGGTTGTCACGTTACCTTTAAAACAAATCATTTTTCTTAAAT23.225806451612903No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT23.225806451612903No Hit
GTCTACTGCAAAATTGATCAGACCCAGAGAAAAGTAGTTGTCAGTCATAG23.225806451612903No Hit
ACTACATGCACCGGACCGGTCACTGGCTGGGCATGGATGTGCATGATGCT23.225806451612903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAAAGGAGGAAATCATATATGCATTTGTCTCAGTGAACACAGGGGTG23.225806451612903No Hit
GGCCTACAAATCGGAACTGGAGGAACAACTGACCCCGGTGGCGGAGGAGA23.225806451612903No Hit
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAAACCCTTGTGT11.6129032258064515No Hit
GTTACCTGCTTCCCCAGTCTTTTCTGAAAAACTACAAATTAGGGTGGGAA11.6129032258064515No Hit
GCATTACCGGAACCGACATTCGCACCGCAAAACTGTACGGCGGTAGAGCT11.6129032258064515No Hit
CTCTAGTCTGAAGGTTAAGAATGACTTGGAGAATACCTATTCCCTGCCTG11.6129032258064515No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.6129032258064515Clontech SMART CDS Primer II A (96% over 26bp)
TTTTTGGACAGAGGGCTCTTTTTGGGGGCAGTGAAATTAAAGTTTAGACT11.6129032258064515No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT11.6129032258064515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.6129032258064515No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAG11.6129032258064515No Hit
TATTAAGACCTCCTAAGTGAAGAGGCATTGGCAGATAGAAGGGATGTGCA11.6129032258064515No Hit
ATTGGGTACTTTTAGTGTCCAGAAGGAAGAGAATACTATATTCAGTGAGG11.6129032258064515No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
GGGCGGCGTCGGAGGACAGGCGGCCGCGGCGACGCTCGCTGGAAACCATG11.6129032258064515No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT11.6129032258064515No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
ACTATATATGCTGAATTCAATCACTTATCACCACCTCCATAACTACCAAT11.6129032258064515No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
GTGGAAAGCAGTGAGTTGAAAACATCAATGCGATAACTGATAAGCCAGCG11.6129032258064515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAC11.6129032258064515No Hit
CCTCTGTGAATGGTGCCGAACCAGAGACCAGAACCGTGCGCCCTCCCTCA11.6129032258064515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.6129032258064515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTGGCACAGCTGTGGGCAAGGGACTGACCTCCGAAGTCAAGCACTCAC11.6129032258064515No Hit
CCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA11.6129032258064515No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGGGGGCC11.6129032258064515No Hit
ATAAAATACATTTACAGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.6129032258064515No Hit
GAATTCCTTGAAGGACTCCTTGTCGAAGTACTGGGAATGCGGGTCGAGCC11.6129032258064515No Hit
TTCAATGATACCATTCGATTCCATTCGATGATGATTTCAATCAATTTTAT11.6129032258064515No Hit
GTCCCTAACAGAATCCACCTCCGAGTTGTGACAATTAAATGAAAAGGTTG11.6129032258064515No Hit
TCATTGCGGTGAATGATCTTCTGCAACTCTTCCACATTCAAGTCGCATAA11.6129032258064515No Hit
GTAAATCATCTTGATGCTTTTGAACCCCCCTATAAATTCCTCTGAACATG11.6129032258064515No Hit
CTAAGTTAGGGATATAATGAAGCCAGAGTCAGGGAAATTACGTCGATACC11.6129032258064515No Hit
CCAAAGTGCATGAGACACATGCTAAAATTACAAATTAAAATTTTGGGTCA11.6129032258064515No Hit
TCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATC11.6129032258064515No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
GCTGTGCTAGCCATTGTGCGATGTGCCCAATATGTATTAGCTTAATTCTA11.6129032258064515No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC11.6129032258064515Clontech SMART CDS Primer II A (100% over 26bp)
GGCCTGGGCCAGTTACCCAAGAAGACAGATGTGATCATCTTACCCTAAAG11.6129032258064515No Hit
CTAGTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATT11.6129032258064515No Hit
GGGAAGCAGTGGTATCAACCCAGAGTACATGGGAAGCAGTGGTATCAACG11.6129032258064515Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAGAGTCATGGGAAGCAGTGGTATCAACGCAGAGTACA11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
TTATCGAGCTGGTAGACGACCAGCGCTGACGAGCTGGCGCAATGACGACC11.6129032258064515No Hit
TCATTTTGTTTGTTTGTTTTTGTCTATTTCAATAGGTTTTTGAGGAACAG11.6129032258064515No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11.6129032258064515Clontech Universal Primer Mix Long (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.6129032258064515Clontech Universal Primer Mix Long (95% over 21bp)
GTCCATGGGAAGCAGTGGTCTCAACGCACCAAAAAAAAAAATAAATGTAA11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)