FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003636214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555301
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG37010.6664853836027668No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA21170.3812346817311692No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG20400.36736832816796655No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG20350.36646791559892744No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC14940.2690432756288932No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC14910.2685030280874697No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA14900.2683229455736619No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC14120.25427650949665137No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC13430.24185081604391132No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT13400.24131056850248783No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT13390.24113048598868003No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG12690.22852471002213215No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG12380.22294215209408952No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG11060.19917126027145637No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT10870.19574969250910768No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA10680.19232812474675895No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG9750.17558045096263106No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT8530.15361038427807622No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA8480.15270997170903708No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC8230.14820790886384141No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA8090.14568675367053183No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT8050.14496642361530054No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT7890.1420851033943753No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT7560.13614238043871701No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC7410.13344114273159963No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT7350.13236064764875266No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC7320.13182040010732918No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA6930.12479718206882394No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA6860.12353660447216916No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG6820.12281627441693783No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG6800.12245610938932218No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6690.1204752017374361No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG6550.11795404654412651No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG6480.11669346894747172No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC6470.11651338643366389No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA6400.11525280883700911No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT6400.11525280883700911No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG6310.11363206621273866No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA6310.11363206621273866No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC6220.11201132358846823No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC6170.1111109110194291No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC6000.10804950828469605No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG5950.10714909571565691No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC5950.10714909571565691No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG5940.10696901320184908No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC5880.10588851811900211No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC5870.1057084356051943No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA5870.1057084356051943No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC5840.10516818806377082No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA5820.10480802303615518No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC5750.10354744543950038No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT5700.10264703287046124No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC5690.10246695035665342No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5620.10120637275999862No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5580.10048604270476733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)