FastQCFastQC Report
Tue 10 May 2022
EGAF00003636267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325506
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT8320.2556020472740902No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6780.2082910914084533No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6540.200917955429393No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC6350.19508088944597027No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA5190.15944406554717885No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5110.15698635355415874No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5080.15606471155677623No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC4930.15145650156986354No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4890.15022764557335347No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4670.14346893759254822No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4450.13671022961174295No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG4410.1354813736152329No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT4360.13394530361959534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.12933709363268264No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC4160.1278010236370451No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG4070.12503609764489748No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT3930.1207351016571123No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT3920.1204278876579848No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA3870.11889181766234723No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG3860.11858460366321973No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3700.11366917967717953No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC3680.11305475167892451No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG3660.11244032368066949No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT3650.11213310968154197No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA3650.11213310968154197No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC3600.11059703968590441No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3580.10998261168764939Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT3560.10936818368939437No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3490.10721768569550177No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT3490.10721768569550177No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC3480.10691047169637427No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC3430.1053744017007367No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT3400.10445275970335417No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA3290.10107340571295152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)