FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGGGGGGTATTTCCTCGAAAGTCTCGGAGCTCAGGATCACAGCCAG53.4722222222222223No Hit
GTTTATTTGATATATTTCAAGGTGGCCATTCAGAGGGACTGGAAACACGC32.083333333333333No Hit
GTAGCGCCGTCCGCCCGTCCGAAGCTCAATGCGCGATGCGCGTACCCAGC21.3888888888888888No Hit
CTATTATATCGATCCACCCAAAAATTCAGGAACACCAGCCTCGCTCCGGC21.3888888888888888No Hit
TTACAGAAAAGTTCCATGTAATTGGTATTGAAAAACCAGAGGTGTCGTCC21.3888888888888888No Hit
GGCAATAACTTAGTAAGCCAACGCTGGTCTTTGTGGGTGCCACGAAATCT21.3888888888888888No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC21.3888888888888888No Hit
GATTACGGGCGTGAGCCACCGCACCCGGCATGGAAGAGGATAATTCTGAT21.3888888888888888No Hit
GAGCGGCTGCAGTCTACAAACTTTGCCCTGGCCGAGTGAGGTGTAGCAGA21.3888888888888888No Hit
GTCTGTCTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGA21.3888888888888888No Hit
TTCCAATGCTGTCTGGAATCAATTTATTGACCACTTCTTTCAAGTCATTT21.3888888888888888No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC21.3888888888888888No Hit
ACTATAAAGAAAGCAGATGTTGGACAAGTGAAAATACGGTAGATAAGTGA21.3888888888888888No Hit
CCTTCTGCTAAGCCAAGGGCTGCCACAATGACACAGTAGCCAGATCCTGC21.3888888888888888No Hit
ACCTTATTCTATGAGGCTGACATCATTCTGATACCAAAACCTGGCAGAGG21.3888888888888888No Hit
CATGGGGAGTCTGCACTGGAGCTGCCTGGTGACCAGAAGTTTGGAGCCCG21.3888888888888888No Hit
ACTCCAGCCTGGGCAACAAGAGCAAAACTCAGTCTCAAAAAACAAACAAA21.3888888888888888No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC21.3888888888888888No Hit
CCCCTGAGGAGATCCGGCGCATAAGCCAAACCTACCAGCAGCAATATGGA21.3888888888888888No Hit
GTATCAACGCAGAGTACATGGGATTCCTGAAGCTGACAGCATTCGGGCCG21.3888888888888888No Hit
GTTTAGGGGCAATAGAATAACCTTTTAACTTTCTCTTTTGTCTCCTGGTG10.6944444444444444No Hit
ATATTAAGCTGGAGATTTTAAGCTGGAAAGGTGTGGTTTGTTTGCTGGTA10.6944444444444444No Hit
ATTTTAGCCAGGATGGTCTCGATCTCCTACAAGATTAGGAGGTCGAGACC10.6944444444444444No Hit
GGGGGAAGTAGTCGTCAAGCACTTGGTTGCACTGGGGCATGAGCAGCTTC10.6944444444444444No Hit
GTACATGGGGGGTCTGGTACATCTTGCCGGTGTAGCCGCGTTCCTTCAGC10.6944444444444444No Hit
CACCAGGAGAGCTTCTCCCAAACTCCCCGTTCCTGCGTAGTCCGCTTTCT10.6944444444444444No Hit
TTATTAACCTGAGGGGGAGTGAAGGGCAGCCTAGGCTTCAACTTGAACAG10.6944444444444444No Hit
CATTTATAGATCAGATGGCCAGTAGTGGTGGACTTGCCCGAATCTACGTG10.6944444444444444No Hit
GTACATGGGAGGCACCTCGGAGATCTGGGTGCAAAAGCCCAGGGTTAGGA10.6944444444444444No Hit
CTTCCTGGGTATGGAATCTTGCGGCATCCACGAGACCACCTTCAACTCCA10.6944444444444444No Hit
AACTAAAACAATGAATAAGCAATTACTTTTTGATGAAAATAATGGGAGGA10.6944444444444444No Hit
GAGTCCTGTATTGTTATTTTTCGTCACTACCATGGTGACCACGGGTGACG10.6944444444444444No Hit
CTGATACTGTTAAGAGTTGCTGTGCCTTTTTGTTTCTTGGTTAATGCTTT10.6944444444444444No Hit
GGTAAACTGAACCTTTGCCTCCAATTACAGGGGGAGGAAACAAGATTGAG10.6944444444444444No Hit
AAAAAATGATAAAGGGGATATCACCACCGATCCCACAGAAATACAAACTA10.6944444444444444No Hit
GTGCAGAGGCCCTGGGGAGCACTTTTGGGCTCCGGCTGCCCCACGGCAGC10.6944444444444444No Hit
CTCCTGAGCATTGACTTCAGCACCCAAGGAAACCAAAAGCTCCACGATGC10.6944444444444444No Hit
CTACTAGCGAGGCTCATGCAGGAGGATTGCATGAGTCTGGGAGGTCAAGG10.6944444444444444No Hit
ACTAAGAGTTGCCTCTTTAGAACAAAAGATGTTCCTATCACCAGGAAATT10.6944444444444444No Hit
ATAAAGCATAAAACAGAGAACATAATTTACCTTTGTGTAATATCTTTGGT10.6944444444444444No Hit
ATTTTAGCCAGGATGGTCTCGATCACCTACAAGATTAGGAGGTCGAGACA10.6944444444444444No Hit
TGTCTGTGGACTGTTCCACCAACAACCCCTCCCAGGCTAAGCTGCGGCGC10.6944444444444444No Hit
CCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCT10.6944444444444444No Hit
GTACCAGCTGCAGTAGCCGCCCTTCTGCCGGATGCAGGCGTAGTGTTTGG10.6944444444444444No Hit
CAGGACACCGAGCTTGTGGAGACCAGACCAGCAGGAGACAGAACCTTCCA10.6944444444444444No Hit
CCATCTGAGAATGAATTTGGTGGTGTGCGCGATATTGACATTCCATTTGA10.6944444444444444No Hit
CTACCACCTCCTTCCCCAGCCTTTCTAGGCACCTACACTACTCTCCTTGG10.6944444444444444No Hit
CCTGGAAGGGGCTGTGGATGTCGTGCGTGGTGCAGAGCACCGGGCACATG10.6944444444444444No Hit
TCTGCAATTTGAGAGTCCTTCAATCCAATGACATTCTGTCCATTGATTTC10.6944444444444444No Hit
ACCCTTGACTCTGTTCCCTTCAAGATCACACACATAGGTCACCACTGGGG10.6944444444444444No Hit
CAGCCAGTTACTTCATGCTTTTCTGGGCCACCCCGGGTCTGAATGGCCAG10.6944444444444444No Hit
ATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTC10.6944444444444444No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10.6944444444444444No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG10.6944444444444444No Hit
GGTTAAGCCAAAACATATGTAAAACAGAAAAAATGCTTTTAGTAAAGTGC10.6944444444444444No Hit
TCATGGTGCTGGGCGCCAGGGCGGTGATCTCCTTCTGCATCCTGTCAGCA10.6944444444444444No Hit
GGTGGAAAATAGATACTGCCTATTTGAGTTCAGAAAAGAGATTTGGGCCA10.6944444444444444No Hit
GTACAGAACACACAATTTAGATGAACTGAAATTATAAGATAAAATAAAAT10.6944444444444444No Hit
GGCTGTCTCTCCACCTCCTCACATTATGCTAACAGGGACGCAGACACATT10.6944444444444444No Hit
TCAGCCCCCACCTCTGCCCTGCCTTCCAACCTTTGAACCACCAGGCAGTA10.6944444444444444No Hit
ATATTTGGAATGACTATTAAAAAAACAACAATGTGCAATCAAAGTCCTCG10.6944444444444444No Hit
TTCCTGCCCTTCCCTTTGTGACTTGAAGAACCCTGACTTTGTTTCTGCAA10.6944444444444444No Hit
GTCTCACACCCTGCAGTGGATGCATGGCTGCGAGCTGGGGCCCGACAGGC10.6944444444444444No Hit
GATGAACCTTCCTTCAGAAAGAGCAGATTTGAAACACTCTTTTTGTGGAG10.6944444444444444No Hit
CTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCATAG10.6944444444444444No Hit
GTCCTACATGAGGCGGCAGGCAGCCCTGGGGGTCAAGAGGAAGTAACTGA10.6944444444444444No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG10.6944444444444444No Hit
AAGGAGTGCCTGCTCATTTCAAACAGGAGCCAATGGTGCATCCAGCTCTC10.6944444444444444No Hit
TTCTTAACCTCAAAGTTTTAAACCTGAAAGGGCTCTTAGTCCAACTTTTT10.6944444444444444No Hit
GGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATC10.6944444444444444No Hit
GCTGCATGCCATGGGGACCTCACAGAGAAGCTCAAACTCCTGTACAAAAT10.6944444444444444No Hit
TCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTT10.6944444444444444No Hit
ATGCTGGACATGTCACTGCAGCTGCCGCCGCCGCAGCCGCAGCCCTTGCT10.6944444444444444No Hit
CATCAAGGTTATGAAAAACTCTGATAAAGAATATAAGGACTTGTAGGGAG10.6944444444444444No Hit
GGTATACCCATCATGCGCTGGCACCACCGGACCACGGTCAGCAACGTCAA10.6944444444444444No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT10.6944444444444444No Hit
GTAGCTTTCTGTATATGTATCATCTGCAAACCTAAGAAGAAGGCCTGTCT10.6944444444444444No Hit
CCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGG10.6944444444444444No Hit
AGTGCTGTGTTGTCTCATAGACGCCTCCAGCAGAGCCAGGGTGCTGCATT10.6944444444444444No Hit
ATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCT10.6944444444444444No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA10.6944444444444444No Hit
ACTTCATGCTCTTCGAGAAGTGCGAGGTGAACGGTGCGGGGGCGCACCCT10.6944444444444444No Hit
TGATTTCACAGTGTGTGGTCAACATTTCTCATGTTGAAGCTTTAAGAACT10.6944444444444444No Hit
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT10.6944444444444444No Hit
AACAGAAGCGGAGCGTAAAGAAAAAGAAAACGCTCATCTTCTGTTAAGCA10.6944444444444444No Hit
GCTAAGGAGTATGGACTTTGGCTTATATTCTAGGGGTTTTCTAATCTGTA10.6944444444444444No Hit
GTCCGAGGAACAGACTCAGCCTCCCTCTCCTGCAGGAGCCGGCATGTCCA10.6944444444444444No Hit
CACTGGATCTCAACGACAGTGGCGGCGTCAAACTCAAATGGCGGCTGGAC10.6944444444444444No Hit
CTGAGGTACTTCCTGGGGTCAGGCAGCAAAGCTATGAGCTACAATTATGA10.6944444444444444No Hit
CTCCACACTATCCTTACTTCTGTCTCCACTCTGATACCAGAGTGCAGCCA10.6944444444444444No Hit
CTACTAGCGAGGCTGATGCAGGAGGATTGCATGAGTCTGGGAGGTCAAGG10.6944444444444444No Hit
CTTCATCTCAGTGGGCTACGTGGACGACACGCAGTTCGTGCGGTTCGACA10.6944444444444444No Hit
TCACCATTCTTTGCAAAAGCCTCCCATTCTGAATCAGTCTGCTGGCAGGA10.6944444444444444No Hit
GGCTGGTCTCAAACTCCTGGCTTCAAGCAATCCTCCCGCATCAACCTCCC10.6944444444444444No Hit
GAACACCCACTTCTTCTTGGCGTCCATCGGCTCGATGATGCGCGCCGAAG10.6944444444444444No Hit
TTACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTTCCTGAATTGCT10.6944444444444444No Hit
CTCCTGAGCATTGACATCAGCACCCAAGGACACCAAAAGCTCCACGATGC10.6944444444444444No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG10.6944444444444444No Hit
CTTTTTAAAGAGATAGCTTCTCTTCTTTCTGAAGATCAATTTCTCCCAAG10.6944444444444444No Hit
GGAATCCCACGCGCAGCAGGCGCCCCTCGTCGGCGATGAGTTCCGCATGC10.6944444444444444No Hit
TCTAGACAGGGTTCGAGATCTCTTGTTGGTTTCTCTCCATCACTTCTGGG10.6944444444444444No Hit
GGACAAAGCCAACCTGGAAATCATGACAAAGCGCTCCAACTATACTCCGA10.6944444444444444No Hit
TAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTT10.6944444444444444No Hit
AGCGGCACCTGATCGAAACCGTTTCGCCGCAGGCCTTCGCTGCGGGCCGA10.6944444444444444No Hit
TCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG10.6944444444444444No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGCTGTCTCTTATAC10.6944444444444444No Hit
CATTGGACAGTGACAGCTTGTTGTTGTTGCACGTGGCCCCTTGAAGTTTC10.6944444444444444No Hit
GTTTACAACTGGCAATTTTCCATGCTCATGGACATATTGAACCAATTCTA10.6944444444444444No Hit
TTCTTACAGTGCTGGATGTTAAACATGCCTAAACGCTTCATCATAGGCAC10.6944444444444444No Hit
CCACGGGAGGCAGCCCAGAGGCCACCGGCACAGGGTGGTGGCCCCCAGAT10.6944444444444444No Hit
TTTTTCTTCAGGATTATCCATGTTTCTGGAACATTTCCACATGTACAAGA10.6944444444444444No Hit
AGAGGGCACTTATTTCTCTCTGTCCTCTCAGTGGGTTACAAATCAGATCT10.6944444444444444No Hit
GTTGGGGAAAGGGAAGAGAAAGGACTGAGTGAGGCTGGGGTGGATGAGTG10.6944444444444444No Hit
AAAATCTTTCCAGGTTGTCGGATTTTCTCCTTCTTGGTCTTATAAAAAGC10.6944444444444444No Hit
ATCATATGTTCTAAAAGAGAAGGAAGAGAAAATACTACTTTCTCAAAATG10.6944444444444444No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGACTAGGACCGCGGTTCTATTT10.6944444444444444No Hit
CTAGAAAATGGGGAAGCTGAGAGAGAATGCCTGATTACTTTTTGTATGTG10.6944444444444444No Hit
TCGCGCAGCATGGCGTTGGCATTGAGCTCCTGCTCGGTGAAGGCGAGCGC10.6944444444444444No Hit
GTCCAAAGTGATGATGGAATTTTTATTCTACTTTTTCATAGATCCGAGTA10.6944444444444444No Hit
TTTTGGGAGGCCGAGGCGGGTGGATCACCAAGACCAGCCTGGCCAAGATG10.6944444444444444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)