FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGAGCGCGTTAAGGCTCGTCCCATCGGAACAAAGCCGTGGATACC36.521739130434782No Hit
AGCTGAGGCCGGGCATGATGGCGAGGCTGACCGCCGCGAGAACGATGCCG24.3478260869565215No Hit
GTTGTCGCGGACAAAGATGCCGCGTCCGGGCCGCAGCGGCAGCGGACGGA24.3478260869565215No Hit
AGCCTGTCTTCACCGGCCCAAGCTTCGTCGATGCCTCCAACGTCGCAGAA24.3478260869565215No Hit
GCGCTGCGGCTCGGCCACCTCGATGCGGCCCGGCGTGTGGCCATGCGAGG24.3478260869565215No Hit
CTGGTAGAGGTCCTCCTTCAAACCCTCCGGCGCATCCAACCGCATCCGCC24.3478260869565215No Hit
GTCGACCACCGCGGAGATGCGGGTCTCACCCACCGTCAGGATGCCCACCA24.3478260869565215No Hit
CTGAGCTAGCTGAACAACGCCCTGAAGCGGCCGCTGCGATGGGCATTGGG24.3478260869565215No Hit
GATTTAACCATCACCCCAAACTCTTCGAATTTGGGGCAAAACGAGCATCC12.1739130434782608No Hit
CCCGCACGTCTTCGAGGCCTTCCGCTTCCACATGACGCTCACCGGCCCCC12.1739130434782608No Hit
GCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAA12.1739130434782608No Hit
CTGTGGGAGCTCGCCTTCGGCGCTCTGCTCGCGATGCTGCCGTTGGCGAA12.1739130434782608No Hit
TGGTGGCTCACACCTGTAATCTCAGCACTTTGGGAGGCCGAGGCGGATCA12.1739130434782608No Hit
CCGGACGCAAGGTCTGCTTGAGCACGGTGCGGACGGCCCACGGGATCAGC12.1739130434782608No Hit
GTACCAGGCGATGGCGTAGGAGCCCCCCATGCCCACCAGGCTGAAGGCCA12.1739130434782608No Hit
GTTCTACGTCCGTGGCTTGGACCGCAACGGCGAACCGTATGAGCTCTTTG12.1739130434782608No Hit
TGTCCGAGGTGGACAGCATCCAGGCGCGCAGGGTCAGCACGGTGCGGCCC12.1739130434782608No Hit
CGGCGCAGGGCGGAGCGGCTCTACGGCATGAGCGAGTCCGACGTCTCATC12.1739130434782608No Hit
CCCCAGCGCCGCGGTGGGCGAATTGCTCGGCACCTTGGCCGCTGCCGGTG12.1739130434782608No Hit
CTACGAGGTCTACGAAATAGTAAGCGAGGTCGTGAACGCGGACGCGGAGA12.1739130434782608No Hit
GGCTACCGCTCGGTCCGCCTGCTCGGGAACGAATGCTCGGAGGAGCTTCT12.1739130434782608No Hit
GCGTAGGACAGAGCCGCCGAGGCGTGCGAAGATTCGGTCCAGTCGTGCTC12.1739130434782608No Hit
ACCTAAGAGCTTCTACACAGCAAACGAAACCGTTAACCGAGTAAACAGAC12.1739130434782608No Hit
CAAGTATTCTTCATCACCGTCAGCATTCCCTTAGTCGCATCCGTAACATC12.1739130434782608No Hit
GTTGTAGATCATGAGGTAGATGACGATCGCAACCGCGGTGCCGATGGCCG12.1739130434782608No Hit
TGACCACGGTGTCGAACCATCTGCCCGAGCCCAGAATGTTGGAGTACAGC12.1739130434782608No Hit
TGATAATTGCGCTCATCACTGCCGGATCCGGTATGCGGTGCATGTATGCA12.1739130434782608No Hit
GCGCCGACCAGATAACTCTGCAACATCACAAAAGCAGTCCAGGCGCCAGC12.1739130434782608No Hit
GCAGAATACTTTGGTAAAATCAATAAAAACTTCGACCCGGAAAAAGTAAG12.1739130434782608No Hit
GTTTGGTTGATGTCTGTACAGAGCCTGACCTCTGCCTGACATTCTCCCCT12.1739130434782608No Hit
CTGCGAAAGCTTCTGTTCAGTTAGGTGACGTTATCCCGTTTCCAACGAAA12.1739130434782608No Hit
CGCGCGTCTCGTTCATCTTGGCTCTGGACGCAGAGATGCCGAACGGCTCC12.1739130434782608No Hit
GGCCGCCCGTGAGCGCCCAGCCCTCGACCGGTTTCAACACCCGCCGCGCC12.1739130434782608No Hit
CACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCA12.1739130434782608No Hit
TCCTTGACGTGACCCTTGAAGGTCTTGGTCGGTGCGAACTCACCGAGCTT12.1739130434782608No Hit
ATTCATTACATCAGACCATTTGGCGGCAGAAAGATCTTATATATCTGGGA12.1739130434782608No Hit
GTGGTGGAGCCGGGCCGCCAGTGTGGGGTACCAGGAGCTAAAGTCAGGGA12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)