FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTAATTCACTCCTGGAGCCCGGTGAACTACTTTGGAGGCTACCGGT33.6585365853658534No Hit
GCTCAGAGAGGTCAAGCTTATTGATCAACATCACACAGCCAGGGAGGGGC33.6585365853658534No Hit
GTATTTTGGAATAAAAGAAGATTTGGCGATTGAAGCCATTTCCGGATATA22.4390243902439024No Hit
GATGGGGACATTGTGGGTGACACCATCTCCAGAGTCCAGCACGATGCCAG22.4390243902439024No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAA22.4390243902439024No Hit
GCGCAGCTGCTTCCGTGCCGGCCGGGACGTGTTCCAGCCAGCCGGAGGGC22.4390243902439024No Hit
GTACATTATTACTTTGCCCATGCCTGGACGGTTGGACTTTGAAGGCATCC22.4390243902439024No Hit
AGATAGAATCGATCGGAATCACGCCGATTGGCTGATCGCCTCTCTTGTTT22.4390243902439024No Hit
GCTGTTAATGCTGTCATTATCATTCTTTTTAAATGAGTATTATGGATCAG22.4390243902439024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAGACAGCAGTCAGGACACACTACAACAAAACGCCTCATTGGCACATGC22.4390243902439024No Hit
GAGTTGCGAAAAGCATTTTGGAAAGCTTCACGCTAACCCTTACACCTATT22.4390243902439024No Hit
CACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTC22.4390243902439024No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA22.4390243902439024No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT22.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTTACTTGTCTTTTCTTGAGTTCAACATTTATGCTTCTAAGAATGCA11.2195121951219512No Hit
CCACTAGCGAGGAGTGGGCAGAAAGCACGGAGAAACAGCGTGTACTTGAT11.2195121951219512No Hit
ATTTGGAATGGAGAACACAGGATGTAATAGACGGAGGTTGGACAAACGAA11.2195121951219512No Hit
CCACAGGCACTGACTGATGTCCTTTTCCATCTCCCATCCTGTGCTGCTCC11.2195121951219512No Hit
GGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTA11.2195121951219512No Hit
CGCGTTCACCCACCAGCGAGTCGTAGCCGTGCGGCAGGCGCAGGATGAAC11.2195121951219512No Hit
CTGTATCAAGAGACAATTATCTTAGGACTGTCTTTTAAAAAACTAAAACA11.2195121951219512No Hit
GACAGGATCATTGGCGAAGGTTATCACCAACAATATGGTAAAGCTCATGC11.2195121951219512No Hit
GAGTAGTGATCTCATCCCTGTGACTAAGCCACACCGACGCCAGGAGCCCT11.2195121951219512No Hit
GCCCAGCTCTCCCACGGCGAGGTGAGCTGAGACTTTCTGAGAACAGTTAG11.2195121951219512No Hit
GAGCAATATTGGGAGCATATTTTCTTTCGAGCATTGACGGAGAACAAGTA11.2195121951219512No Hit
GTATCATGTGGGCCTTTTGAAAGTAACAAACAGAAGGCCAGTCTGTTGCA11.2195121951219512No Hit
CTCATAATTTATTAACTAATGTGAAATGGAAGGTGAAAGAGGAATTATGA11.2195121951219512No Hit
TAGACAGACCTACAGTTAGTATCATAGTGAATGGGAAAAAAACTGAAAGC11.2195121951219512No Hit
GCTCAGAATCACCGTCCTAAAAGGCTGTCTACCAAAGAGTCAGGAAAGCA11.2195121951219512No Hit
CCGACAAGGCTGTATCGTGCATATTGACAGTGCCCGCCTTGTCCTGATCC11.2195121951219512No Hit
TCCTGAGAGAGGCCCGAGAGTGCCCTCTGAAGAGGAAACCGCATCACCGA11.2195121951219512No Hit
GGTATATACCCAGTAATGGGATTGCTGGGTCAAATGGTATTTCTATTTCT11.2195121951219512No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT11.2195121951219512Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGAAAAAAAAAAAAAA11.2195121951219512No Hit
TCGCCCAGCAGGTGGCCAAGGCTGTCGTTGACGACCTTGAAACGATCGAT11.2195121951219512No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
TCCGGGCGGTGGGCGAGGCGGAGGTGGCGCGGTCGATCATGGCGATTGTC11.2195121951219512No Hit
ATCAAAATCAGGCAGAGATCACCGGGCTGCAACCGCCCCAGCGCCGTATC11.2195121951219512No Hit
GTGATAAGGATGTTTTTCATTAGTCATTTCAGTTGGTGTTGAGCAAGGAT11.2195121951219512No Hit
ATTACAGGCGGGAGCCACCACCCTGGTCAGTAGTGAGAACTTTGTATGTC11.2195121951219512No Hit
TTTCTATGGTTTATTGGCCAGCACATATTTTCTGTTGTGAGTTTTCCTTC11.2195121951219512No Hit
AGGAGGAACCGGGGCTCGGCAACGGCGGCCTCGGGCGGCTCGCGGCGTGT11.2195121951219512No Hit
GTTATTATTTGTCAGGACCTTCCGCAAAGGCCTACCTGGATGAACGCCTT11.2195121951219512No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAA11.2195121951219512No Hit
ACATAAAGCAATCTACAGATTCAATGCAATCTCTATCAAAACTCCAGTGT11.2195121951219512No Hit
ATATAATGTTGTTATGAAGATTAAGTGAAATATATCAATATCTAAATCTA11.2195121951219512No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
ACTATGCTTTTCTCTATTTTCCCGAAAAATCCACGAAAATCAAGCCATCC11.2195121951219512No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC11.2195121951219512No Hit
GCTTAGTGTTGCGCGATATCTCGGCCCGCAACACGCTGGCGGGGGCGCGT11.2195121951219512No Hit
CTTCTTGAGGTACTCCATTTCGGTAGGCTCATCCTTGGCCATCGCCAACA11.2195121951219512No Hit
CACGTGCTGAAGCCAATGATGTGGCCAACGCCGTATTAGATGGGGCGGAC11.2195121951219512No Hit
TTTTTGTAGACATGCAACGCTGTATCGGTTGTCATGCCTGTGAAATGGCC11.2195121951219512No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA11.2195121951219512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT11.2195121951219512No Hit
GCCCGGCGGGTCATGGGAATAACGCCGCCACATCGCCGGTCGGCATCGTT11.2195121951219512No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAATGGTTTTTAACA11.2195121951219512No Hit
AGTGGTTGCTGCGTCCATTTGGCCAGCCTTCGCGGTGTTCTTTGTCAAAA11.2195121951219512No Hit
GCGCTGGTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGG11.2195121951219512No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATA11.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
GTTATGAACTGTTATTCCATTGGGGCTCAAAGTACCTAAGGGTGCAGTGT11.2195121951219512No Hit
ATTCCATGCAATTCCATTAGATGATGACTCCTTTCATTTCCATTCGATGA11.2195121951219512No Hit
GGAAAGACAGAGGAAGGGAGGAAGGATGGGAGGAAGGAAGGAAGGAGGGA11.2195121951219512No Hit
GGGTTGGGTGATCCTACGATGGATATAAGCTTTATAGGAATTACCAATAC11.2195121951219512No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)