FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACAGGCGTGAGCCACCGCGCCCGGCCTAGAGGCAGGATTTGAACCT33.6585365853658534No Hit
CCTCTCCACCTAGCGAAACATAGGTGCTGCCATTTTCGGACAGCGGCATA33.6585365853658534No Hit
ATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCGGGGGGAGTATGGTTG22.4390243902439024No Hit
GCCTAGGCAACACTGCAAGACCCTATCTCTACAAAAAATTAAAAAATTAA22.4390243902439024No Hit
CAGTAAGGTTACAAAGGATAGCGGAATCCCGATACCTATCATCAAGGACG22.4390243902439024No Hit
ATCATTTTGTGCTTCTTCCCGGTCATATTCCCGAAAGTCTTAATGGCTTC22.4390243902439024No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
TGCCCGGGCACCCGGATCCGGGCAGATCCGCACCGCAGTTCCCGCAATAA22.4390243902439024No Hit
CCCTGAAGTACCCGATAGAACATGGCATCATCACCAACTGGGACGACATG22.4390243902439024No Hit
GTCTGTGGCATATACATGTCTGGAAGATGTTTGTTGGACTTGAAACAGAC22.4390243902439024No Hit
CGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCT22.4390243902439024No Hit
ATGTAAAGTGCCAAAACCCCAAATCAAATCGATGGCGGACAGGCCGATAA22.4390243902439024No Hit
CCCTGGTGCCGCTGTAACATCAATCCAGATAGATGGTGTACTGCACGAAT22.4390243902439024No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT22.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTCTCTCAATATTGCTTTGGC11.2195121951219512No Hit
ATTATCTTCCATATCAACGAAGGTAAAGAAGTCTAGAATGTCAGGTTTCA11.2195121951219512No Hit
GAGCAGACCAATGTAAAATGTTCAGCAGGTTTTCCAATGTCTCTTGGATA11.2195121951219512No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA11.2195121951219512Clontech SMARTer II A Oligonucleotide (100% over 22bp)
TCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACC11.2195121951219512No Hit
CGATGGTGCCCAACGAATACCTCCAGCCTGCTGGGGCTGGAGCTCCTGTC11.2195121951219512No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.2195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
CGCCACCACACTTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCA11.2195121951219512No Hit
AGTAAGACCTAAAACCATAATAACCCTAGAAGACAACCTAGGCAATACCA11.2195121951219512No Hit
GGATCTAGAAATAGAAATACCATTTGACCCAGCAATCCCATTACTGGGTA11.2195121951219512No Hit
CTATCTACCCTCCTTTAACTGCGGACGGCGATTAAATGCATCGATTCGCT11.2195121951219512No Hit
CTGTGTTACAGAAGAGGAAACTGAAACCAAGAAAAAAATAAGAGCATTGC11.2195121951219512No Hit
GGGCGCGATCGAACTGCGCGACCTGGGCTTCCGTTATGGCAACCGCGCGG11.2195121951219512No Hit
GATATAATTTCCCATGTATTGCTTTTGCCCCCTTCAGTGAACCTCCCGGA11.2195121951219512No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGGCGTTTATAACAC11.2195121951219512No Hit
ACCCATTATAAACAGGTAAACAGCCACATATGGTTTTACTTGTTCTCCGT11.2195121951219512No Hit
ATTTTCGTCGTCCTCCGGAATTTTGTAAAGGTTGTGATAGATTTCGGCGT11.2195121951219512No Hit
ATCCATTTTAGAATTAGGGGGATAGAGGCCTGGGTTTGCCTAAAACTTCA11.2195121951219512No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.2195121951219512Clontech Universal Primer Mix Long (96% over 26bp)
GTCATGGTGTGTTACTTCATCCTATCTATCATTAACTCCATGGCACAAAG11.2195121951219512No Hit
GAGTCCATGAAACACGCCGCGAGCCGCCCGAGGCCGCCGTTGCCGAGCCC11.2195121951219512No Hit
GAATAAACACAGGTCTCCAGTACAATTCATCCTGTAGGCTTGCCTTGGGT11.2195121951219512No Hit
TTCTTGGCGGTGTGTTTGACGACGTCGTGCTGTATGAAGATCAATGCCAG11.2195121951219512No Hit
GATCCTGTACCGCGACGATCACCTTCTTTACCTGCTTCTCAATAAGCAAT11.2195121951219512No Hit
AAACAAGCTCGTATCTCTCGTATCCTGCCCGAGCAAGTGCCATTTCGGTT11.2195121951219512No Hit
CTCTTCATATAGATGATGAAATTCTTCAGCTGGAAGATTCGTCGGATCCT11.2195121951219512No Hit
AGGGTGCCGTTACGCATTACGTCGGCATTCAAGACGACGTGACCGAGCAG11.2195121951219512No Hit
ACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTA11.2195121951219512No Hit
TCTACATTCTAAAAACCCCCTCTATGGAATCTTAGGGGGATTTTTAGCTG11.2195121951219512No Hit
AGCAATCCACCCTCTTCGGCCTCCCATAGTGTTGAGATTACAGGTGTGAA11.2195121951219512No Hit
ACATAACTCTTAGGACACTAAATAAAGCACTAACCAAAAAAAAAAAAAGC11.2195121951219512No Hit
GTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG11.2195121951219512No Hit
GCACAGATCTCCCCTGCGACCATGTCTTCCATTAAGATTGAGTGTGTTTT11.2195121951219512No Hit
ATGGGAAGCAGTGGTATCAACGCAGAATACTTTTTTTTTTTTTTTTTTTT11.2195121951219512Clontech SMART CDS Primer II A (96% over 26bp)
ATCGTGTCATTTGCAAACAAGGATAGTTTGACTTCTTCCTTTTTCCAATT11.2195121951219512No Hit
TTCTTTATCATGTCTGTTTTATCCAGCTTAGTACACGTGTACAAAAAATC11.2195121951219512No Hit
AATGAAAACATTCTTGGCAAATGCTTTCTCTCTGGTCCGTCTTGCGCCGG11.2195121951219512No Hit
GTGGGGAAGCGGCTTTGTTGACTGCTTATTATCTGGTGGCCAACGAAAAC11.2195121951219512No Hit
AGAAAGAGCACCGCCTTGATCGCCAAGCGCATTGGAACCTGCTCCGCCGT11.2195121951219512No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA11.2195121951219512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGATGGATAGCATCTGCAGGACAGACATTGGCGCAAGCAGGGTCTTCGC11.2195121951219512No Hit
GATCAGGACAAGGCTGGCACTGTCAATATGCACGATACAGCCTTGTCTGA11.2195121951219512No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.2195121951219512Clontech Universal Primer Mix Long (95% over 21bp)
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA11.2195121951219512No Hit
AATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGG11.2195121951219512No Hit
GGAGATCGATACCATCCTGGCTAACACGGTGAAACCTCGTCTCTACTAAA11.2195121951219512No Hit
GGATATGTGTCCTTTTGTGGACACCAAGACCATTTGGCCAACAGCTATCC11.2195121951219512No Hit
GAATAAGATCATGGACTTATTCTACATCGCCAGATGTTGTTCATCTTCTA11.2195121951219512No Hit
GAATAATCATTGAACGGAATCGAATGGAATCATCATCGGATGGAAATGAA11.2195121951219512No Hit
CTGTTGGCACTCTCCAGGTTGACAAGGAATTTCCTGGTATACCGCGGGTT11.2195121951219512No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCC11.2195121951219512No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)