FastQCFastQC Report
Tue 10 May 2022
EGAF00003636499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444075
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT12110.2727016832742217No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA11320.2549118955131453No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC8770.1974891628666329No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT8730.1965884141192366No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT8700.1959128525586894No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT8210.18487868040308506No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG8180.18420311884253787No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC7750.17452006980802792No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT7290.1641614592129708No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC7190.1619095873444801No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC6960.15673028204695152No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT6700.15087541518887576No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6700.15087541518887576No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT6600.14862354332038508No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC6560.1477227945729888No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC6450.14524573551764905No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC6410.1443449867702528No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6390.14389461239655463No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA6380.14366942520970558No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6330.1425434892754602No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC6260.14096717896751673No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT6220.1400664302201205No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC6180.1391656814727242No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC6150.138490119912177No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6080.13691380960423352No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6060.1364634352305354No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG6000.13511231210944097No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG5990.1348871249225919No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT5980.13466193773574284No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT5960.1342115633620447No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG5760.12970781962506334No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA5760.12970781962506334No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG5730.12903225806451613No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5590.12587963744862918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5450.12272701683274223No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC5430.12227664245904409No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA5380.12115070652479874No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT5280.11889883465630804No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC5110.1150706524798739No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA5010.1128187806113832No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC5000.11259359342453415No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC4940.11124247030343973No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC4940.11124247030343973No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG4900.11034172155604346No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC4790.10786466250070371No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG4760.1071891009401565No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC4750.10696391375330744No Hit
GAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGG4720.10628835219276024No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT4710.10606316500591116No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA4680.10538760344536396No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC4640.10448685469796769No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC4570.10291054439002421No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT4570.10291054439002421No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC4540.10223498282947702No Hit
CCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCC4510.10155942126892979No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG4500.10133423408208073No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC4480.1008838597083826No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG4450.1002082981478354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)