FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGATGTATGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGC23.076923076923077No Hit
ACTTTACTCTAGCCTGGGGTGACAGAGACCCAGTCTCTTAAAAAAAGAGA23.076923076923077No Hit
GTCAGAGGGGAGGGCGGCCGGCAACGACACCTCGGGCTCGAGGGCCTCCA23.076923076923077No Hit
AGTGGGGCCAGGGGCCGGCCCAGGCCAAATGAGTGCCCTCCTTGTTATGA23.076923076923077No Hit
CTCCAAAAGGGTGAGCACGGCGCCCTCGCGCACGGGGCCTTTTACATTGC23.076923076923077No Hit
GACCCTGACCGGCAAGACCATCACTCTGGAGGTGGAGCCCAGTGACACCA23.076923076923077No Hit
GGCTGTGACTGCTGAAAAATGCTCATTGGCCCTTCTCGAGGACTGAAAGG23.076923076923077No Hit
CTTCACACCTGTCTGCTGCCATGGGTGCAGAGCCCTAGTCCTGATGGCCC23.076923076923077No Hit
GTCATCAGCGCCAGCAGCAGGAGGTGATCCAGCTCCTCCAGGGGCTGAGG23.076923076923077No Hit
CCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA23.076923076923077No Hit
CACACACACACACACACACACACACACACACACACACGCACACAAACACA11.5384615384615385No Hit
CAAGTCAGCCTGTCTGAAGCTACCACAGCAGGCCACCTCTGGTCCCTGGT11.5384615384615385No Hit
CATTTCCACCTGGACACCTGACCATGTGCCTGCCCTGAGCAGCGAGGCCC11.5384615384615385No Hit
GGCTAGGGCTATTGGTTGAATGAGTAGGCTGATGGTTTCGATAATAACTA11.5384615384615385No Hit
GCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGC11.5384615384615385No Hit
ACTGAAAAGTCTGCACAGGTAGGAATTCAGTAGAAAAACAAAATGGATTA11.5384615384615385No Hit
TTCCAGAGTTTTCCAGGGCCACTGCTGAGAAGGTTAACCAGGACATGTGC11.5384615384615385No Hit
GGACCCACAACCCCTGCCCATGAAGACCTGTGGAGCTCAGGGCATCCCCT11.5384615384615385No Hit
CCTCCAAAGGCAGGACTGGACTAATTCCAAGCAACTATGTGGCTGAGCAG11.5384615384615385No Hit
GAATTTCAGAGCCAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAA11.5384615384615385No Hit
GCCTCTTGGCCTTCTGCCCAGCTCCTGGCTGAAGCTGTGATCTGTGTAGC11.5384615384615385No Hit
TGTCTCCACTTTGATGAAAATGCCACATAGTGTCAAAATAGTGACTGAAG11.5384615384615385No Hit
GTCGTTGGAGACTGAAGAGGAAGAGGAGGAGGAGAAGGGGAGTGAGCGGC11.5384615384615385No Hit
GCTCTACAGTGCTCAGTCTTTGAGGTCACGAGAAGAGAATGCCTTCTAGA11.5384615384615385No Hit
CATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACG11.5384615384615385No Hit
TGAGGGCCATGTGCAGTGTCTCACGCCTGTAATCCCAGCACTTTGGGAGG11.5384615384615385No Hit
CCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCAG11.5384615384615385No Hit
CTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGGTTTGCTGTGAAC11.5384615384615385No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11.5384615384615385No Hit
GGGTCACTGAGCAGCTCGAAGTCGGGCAACGACACCCCATCCAGGTAAGC11.5384615384615385No Hit
GTTCTGGGTTTCCACCTCCAAACCGCTCCCCAAAGACTTTGCCCAGATCT11.5384615384615385No Hit
GGTTAAGAGATGCTCCCAACTGAAATTCATGATATAAACTTGAGTGGGCA11.5384615384615385No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT11.5384615384615385No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGATTTCTGGTATAAAAATCTGTCTCT11.5384615384615385No Hit
GGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACA11.5384615384615385No Hit
GCATTTGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC11.5384615384615385No Hit
GACCAAAGCCCATGTCATCATCAGACTCCTCGGATTCTTCTTTCTTTGCT11.5384615384615385No Hit
GGGTGTGAGACCCCTGGCGTCCCTGCGATGGAGATCAGATTCAGCTGCCA11.5384615384615385No Hit
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAG11.5384615384615385No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGATAGTGTGACA11.5384615384615385No Hit
GTCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACC11.5384615384615385No Hit
GCCTGGCTTCTCCCTGCTCCCAGTGTCTGCTCCTATCTCAGAGCAAGATT11.5384615384615385No Hit
GTGGTGGATGTGGTGGTGGTGACAACCGGACGTGGTCGGCGGCACGTCTT11.5384615384615385No Hit
CCACAGCTCCGATGACCACAACTGCTAGGACAGCCAGGCCAGCAACAATG11.5384615384615385No Hit
CGCTTGAACCCGGGAGGCGGAGGTTGTAATGAGCTGAGATCACACCATTG11.5384615384615385No Hit
CAGTAACACTACACCAATGCCTTGGCATTGGCTCTGCCCATGTGGAATTA11.5384615384615385No Hit
TCTTAATTGCCACCACCTCATTGGTGTGAGCATTTGTAGCAAAATAAACT11.5384615384615385No Hit
CCTGTAGGTCTTTCTGGTGACCTTCTATAATTGCTGCTATAACCTTTACC11.5384615384615385No Hit
GTCTTGTCTCCTCCACCGCCTCCCGGTCCTGCCTCTTGGAACCCCCAGCC11.5384615384615385No Hit
CTTGGGCTCTAAGGACCCAGGAGTCTGGGTGCACAGCCTCCTTCTCTCTG11.5384615384615385No Hit
GTACTGGGAACGCCGAAGGTGGGAGGCCATTTCATAACATTTCTTGTTGA11.5384615384615385No Hit
GAGACAACCTCACCCTCTGGACGAGCGACCAGCAGGATGAAGAAGCAGGA11.5384615384615385No Hit
GTCCAAAGGCATCGTGGTGACCGCCTACAGCCCCCTCGGCTCTCCTGACA11.5384615384615385No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA11.5384615384615385No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)