FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCAAGGAGGACGAGGCCAGCATGTCGCGCGTCCAGTCGTCGCGCGGC42.2988505747126435No Hit
CTGTAGGGCGTCGCGCGCGTCAGCCGCGGCCCGGGCGCGGCGACGCGCCG31.7241379310344827No Hit
TCTTTGAGGCCTTCGTTGGAAACGGGATTTCTTCATATTATGCTAGACAG31.7241379310344827No Hit
AATTGGCAGATGGCAGCAGGCAAAAGACAGGATGGAGTCTGATTGACTCC31.7241379310344827No Hit
CCCGTTCAGTCCACGTCCAATACCGGCATTGAGCGCATAGCCGGCCAACT31.7241379310344827No Hit
GTTTAACTTTTAGGAGGTAAAACATATGGACCAGAGCTTAGAGGACATCA31.7241379310344827No Hit
TCTGTGAATTGAGGGCATACATCACAAAGAAGATTCTGAGAATGCTTCTG31.7241379310344827No Hit
CGCTATGACGGCCCGGCGCATCTCCTGACCCTTGCCCCCACCCGCGCGGG31.7241379310344827No Hit
TCTTCATACTCTTTCAACTTGGCGCCCAGTTCTTCGCCCATACGCTTCAG21.1494252873563218No Hit
CAGATCGGTGGCGTACGTTTCTTCCACACTGACCGACTTGCGGATCTGCG21.1494252873563218No Hit
GCTAAGTACCTATCCTTGCTTTGAATGGATATCTGAAACTGTTTTCTGTC21.1494252873563218No Hit
TAGCGCGCCAGATCGCTCGGCAGCTCCTCCGGCAGCGAGAGGTGCTCGGC21.1494252873563218No Hit
CAGCGTCAGATAGCGCTGGTGCAGGCGGGCGACATCCCCGGCGAGAGTAT21.1494252873563218No Hit
GCTTCGGCCGGCTGCGGGTCATGGTGGCGCAGCAGGACGACCTGATCGGG21.1494252873563218No Hit
CCATCTGGGTGCCGGGCACTTTGGCGCCGAATCTCAGAGTAACTCCCTCA21.1494252873563218No Hit
CTCATGCCGAGCGCGATGCGCCGCGGCTACGTGTTGGCAATTCTGGCGGC21.1494252873563218No Hit
ACTTTGTGATGTGTACATTCAACTCACAGAGTGGAACTTTCCTCTTTATA21.1494252873563218No Hit
GGCTGATAGTACCTTAGGTGGGTAGTAACCAGTGTGGTTTAAACCACTCA21.1494252873563218No Hit
CTGTAACCACTGCCTCCCAGGTTCAAGTGATTCTCCTGCCTCAGCCTCCC21.1494252873563218No Hit
GTGCCGCTTCGCCGAACGCCAGGACCAGAACGGGAATGCCCATGTTGCCC21.1494252873563218No Hit
CACAATAACCACTGGAGCTTCAGTCTGTTCAGATTAGAAGGTGTTCAATA21.1494252873563218No Hit
ACTTAATAAATATGAATTTGCAGCTGCTTCGTCTCTATCCCCACCTCCCT21.1494252873563218No Hit
CCCAGGAAGGGGACCCTGCAGGCTCGGTGATCCCAACTGGATGATTTCGC21.1494252873563218No Hit
CTTCTCACGTTCGGCGGTGCGGATCGCCTGGGTCAGACGGTCTTCCAGCA21.1494252873563218No Hit
CCTTTCACCACTTTCCGGCAAATCTTCGAGATTTCACGCTCGAGATTGCG21.1494252873563218No Hit
CTTTGAGACAGAGTCTTACTCTGTCACCCAGGCTGGAGTGCAGTGGCACG21.1494252873563218No Hit
CTTGCCAAGACGCAGGCGTTCGTCATCGACATTGATCGGATGCTGTCGCA21.1494252873563218No Hit
TAGTTGCCCTTGCCTTCGGCGACCTTCGCAATGACGACGGACGGCTCTTC21.1494252873563218No Hit
AATGTGGGTTGCGTGAATGGAACAAATAGGGTACAAAATTCCTATGGAAG21.1494252873563218No Hit
AAGCAGCATAATCACCCATAGACACCACTACAGGCTTCTTGCTTCTGAGT21.1494252873563218No Hit
TCATTGATGGCATTGCCTCCGCCGCCCGTCTCTCCCCCGAACTCTTCCAC21.1494252873563218No Hit
GGCCATGGCGGCGTTCATTGAAACCCACGCCTCGAACGGAATGCCGAGCG21.1494252873563218No Hit
ATGTATCCAGAGATGGAAACTACTGCTATTCTCTCATTCTAACCAAGGTA10.5747126436781609No Hit
GTGTTTGCGGGCATTGCGGGCAGCTACCTGTTGCGGTCTTTGCTCACTAA10.5747126436781609No Hit
CTTTAGGTTTTTCGACTGCGATTGTCACGCAACTTTCCTTGGCTGAAGTT10.5747126436781609No Hit
AGCTTGGGCTGGTTGCGTGCCACCATGTTGTCGGGCTTCATGCGGAACAG10.5747126436781609No Hit
GCTGATATCGTCCTTCGGCAGGCGCAGGATCCGGGCGATGTCCTCGACGA10.5747126436781609No Hit
CTCGTGCGCGATTCGGAAGGCAAGAAGATGAGCAAGTCCGAGGGCAACAC10.5747126436781609No Hit
TGATCTTCGTGATCCCGGCGTTCAAGGAAGTGTTCACCTCATTTGGTGCC10.5747126436781609No Hit
CAAGTAAGCTGTTGCACCGCAAGTTTCGCACCAAAACGGTGCAAAACTTG10.5747126436781609No Hit
TCCCCACTGACATCATGCTCCAGTTCCTGCTTGGATTTACATTGGGCAAT10.5747126436781609No Hit
CTGTTGCATAGATCGACTCTTTTTCTTTTTTCATAGACGATTTCTCGGTA10.5747126436781609No Hit
TAATTGATGGCATTGCCTCCGCCGCCCGTCTCTCCCCCGAAATCTTCCAC10.5747126436781609No Hit
TCTCTGGGTGCACGTCATGTTCTATCTTAACACATTTGCTAATTTAATTT10.5747126436781609No Hit
CTCCAAAAGAATGTTTTCAATGTTTTCCCTTCTTTTTTTTTTCCTCCCCT10.5747126436781609No Hit
ACCAATAGTTGTTTTCCCACTACCTGACTCACCTACAAGCGAGAATGTTT10.5747126436781609No Hit
TGAAGATGCTGGATCCCAGCATGGAGAAAATTCATATTTTCCCAGTGTGT10.5747126436781609No Hit
GGGCGGCGGTAGGCTGCGGTGATGGCCGCGCGCAGAACCGACTGCGGTTG10.5747126436781609No Hit
CTCCAGGAGCACTGAGGTTGGGGCGACCTGCTGCCAACTGGTCTGGTTTT10.5747126436781609No Hit
GGGCACCAGACTGCTCCGCCGACGTCCGGGGGCGAGCCCCGCCGACCATG10.5747126436781609No Hit
GGCTGGCTGTACGTGGCGTTCGTCATCGACGTGTTCGCGCGGCGCATTGT10.5747126436781609No Hit
TGAGTGTCCAGGGTAACCCTGGGTGCCTGGGGCTGACTGTGGCAGAAATC10.5747126436781609No Hit
TGATGGGAATGTACGTGGCCATTTACGGCCTGCAGAGAATCCGTTTCTTC10.5747126436781609No Hit
GAACGTGACCAAGAAAGCCATCATCGAACGCATGAAGGAGATCGACGTCG10.5747126436781609No Hit
AGTTGAGCAAACAGGACAGCTCGAGCCGAGGCGAGGGGACGACGCGCCCA10.5747126436781609No Hit
GAATACATGTGTTCTGTGGGCCCCCAGGCTGTTTTCGAGCTGGAGCACAT10.5747126436781609No Hit
ATTCTTGAATGAGGCCCTGCACCAGCCCTTCACGGCCGGTGCCGACCTTT10.5747126436781609No Hit
CAGCCATCGGCGGGCGGCGGCGTGTCAAGAAATATCTATGGAATTGTCAG10.5747126436781609No Hit
ATTCCATTGTACATATACACCACATTTTGTTTATTCATTCATCTGTGGAT10.5747126436781609No Hit
TTATTTACATTGCTTCCACATTCACCCTATAAAATCCTTTCCCTCTGCCC10.5747126436781609No Hit
GGCGTCATGCGTTACTCGGCCCTCGCGCCCGAGTCCTTGGCAACCTTGAT10.5747126436781609No Hit
ATCCTCTTTTATTTCCTTGAGCAGTGGTTTGTAGTTCTCCTTAAAGAGGT10.5747126436781609No Hit
ACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGGACGCGGAGTTT10.5747126436781609No Hit
GCTACAGTCATTGAGTCTGCAGGTATTATTCAGGGTAGCATGGTTACTGT10.5747126436781609No Hit
GACACGAGTGCCCCGGGAAATGCAGGCGCCGGTGCTGGCCATTAACCGGT10.5747126436781609No Hit
CGCGAAGGGTCCGAATCCGTAGCGATATTGATCGTGTGAGGAGAACCCGA10.5747126436781609No Hit
CTTCAGGATCTCTTTTAGAGCAGGCCTGGTGGTGAAAAAAATCTCTCAGC10.5747126436781609No Hit
GTGATGTTTTTATGTGAAGATAATGCTTTTCAACCATAGGCCTCAAAGCG10.5747126436781609No Hit
GGCGCGGATCGCCCAGGAGCCGGACAAACGCCGGGATCTGCTTGACTGCG10.5747126436781609No Hit
AGGTTTCTTTGAATACCTGGAAAGTGTTCCCAAAGATAATGGGCACAAAC10.5747126436781609No Hit
GCAAAATTCTGGCCTTGGTGTTGCATTAGCAACAGCTCACTTTGCTCCTG10.5747126436781609No Hit
GAGTTAAATTAATGATTTTATGAAACACAGGTCTGAACATTACACACATA10.5747126436781609No Hit
GAGTAACTTACTGGCTGGGCGCGGTGGCTCATGTCTATAATCTCAGCATT10.5747126436781609No Hit
GTGCAATTAGATTTGGATGACAGTCATTTTACTGCTGGTTTATTAGACAA10.5747126436781609No Hit
AAAATCCCAGTAGCGCCGCTGCAGAGCGAACCAATCGGGGGCGATACGGC10.5747126436781609No Hit
GCACGGTGGCATTCATCCACGACAAAGGCCACTCGGAGTCCCTTGATTTT10.5747126436781609No Hit
GAGCTTGGTTGGGACACCTGTTAGAACCTTTAGGTTATGAAGTAGATGTT10.5747126436781609No Hit
CCCGCCCGAGACCGAGACAGAGCGCTTCGCCGCGCCGATATGGCTGCACA10.5747126436781609No Hit
TAAGCACCCAGAACTCCGGAGCGGGGAACTTGGGGTCGTGTTTCATCAGT10.5747126436781609No Hit
CCACTGGCCAGCACAGCAAAGCCTAAAACAACAACAAAACTAACCACTCC10.5747126436781609No Hit
CCCCAGTACTGTCAGGGTGGTATCCCAAGGTTGACTCCACTAAGGCTGAC10.5747126436781609No Hit
TCTATACACAACATACACCACAGTCAAACACTCAAGGGAAAAAAATATAA10.5747126436781609No Hit
GTGCGACCCAGCGGGTGGCTGGCTCCAGGTGTGCCTGACTATCGACAATC10.5747126436781609No Hit
GTCTTTGCGGACGATCAGGTCCGGATTCCGGAATAACCGTGCCACTGGCC10.5747126436781609No Hit
CTCAAGGCATCGATCCGGTTGTAGCGCGCATTACGATTTCTTTTAAAACT10.5747126436781609No Hit
GCATTTCATCGACACGTTTGGCCTGCGCCGGGCTGATCCGGCCGGTATAA10.5747126436781609No Hit
TCTCCGCAGCCTGCGCGAACAGCCTTAACGTGCGTGCCGTATCGTTATCA10.5747126436781609No Hit
CCGCGTCAGATAGCGCTGGTGCAGGCGGGCGACATCCCCGGCGAGAGTAT10.5747126436781609No Hit
AAGTAGCTCTCTGTGTCCAGTATTGGCAGAAGCAGCAGAAACCTGTGGCC10.5747126436781609No Hit
GAGTATCAGTAACGACGAGGACAGCGATGAGAGCGACAACCAAGGTGCCG10.5747126436781609No Hit
TCTCCGCAGCCTGCGCGAACAGCCTTAACGTGCGTGCCGTATCGGTATCA10.5747126436781609No Hit
AAGATTTCCAGGCTATGGCCCTTGACCCGCAGGAAGTCCGGCGTCAGCGT10.5747126436781609No Hit
TGGATGCATCGTTGCCCACTGTGACCCTGATGTGGTGGGTGATGATGACC10.5747126436781609No Hit
TCCTTGAACTCCTGGACTCAAGCCATCCTCCCACCTCAGCCTCCCGAGTA10.5747126436781609No Hit
ATTTCACGCTCTTCGTGCTTCGGGCGGCAGAACAGCACGCTGCGCGCCGG10.5747126436781609No Hit
ATCCCATACTTCTTCCTCTCTCAATCTCATGGCTGCTACCTTGAGGCTAT10.5747126436781609No Hit
GATCGGGAGGGCGCCGACTTGGCGATGCTGTTCGGAGCTGTCAGTAAGCG10.5747126436781609No Hit
GCCGAGCGGACGCGCTGGGAGATGACTCGGGGTCGGACCGGACGCATCTG10.5747126436781609No Hit
AAGTATGAGTGTGGCCCTCATGAGTTCTAAGGTTTTTACCTTCCTCCTTA10.5747126436781609No Hit
CTTTGGATCATTGCAACAATTAAGTCTAATTTGATGAGTTGGTCGGAAAG10.5747126436781609No Hit
CGGCAGGCTCGTGCGGTGCAGCCTGCGCACACTGGCGACCTTCTCCTGGG10.5747126436781609No Hit
AGATCGCCCAGATCCTCTCCACCTGGGATCAGGCCATCACCGCCACCGAG10.5747126436781609No Hit
TAGCCGGGACCTTGCGCGTCATCCTGCGGCAGCAATGCGAGCGGGCCTTC10.5747126436781609No Hit
TTCCAGGGTGCAGAGACTTGTGCTGGGCCCACTGTCTGGCATGCCCTAGT10.5747126436781609No Hit
GTGTGGATGTCCAAAAAAAGTATTTATTTCCCCTTTGAAACACAACATAA10.5747126436781609No Hit
TGTCTGAAATCGAAGCCGTGTGCCAGCACCTGGCGGCGCTCGACCCCCTG10.5747126436781609No Hit
AAATTCCTGTTGTGGAACAAGGCTGGACGGTGATGGGAATGCTCAGGATA10.5747126436781609No Hit
GTTGCTGATCTCGCTCAATGTGAAGAAGTTGTTAAACGCTTAAAAGCAAT10.5747126436781609No Hit
CTATTGGTGACCTGCTGTGCTCCAGCAGCACACAGGGCCTTGCCAGGCCT10.5747126436781609No Hit
GGGATCCCCGGAGTTGGAATAAGGACCGTGGCATCCATACCGAACATATG10.5747126436781609No Hit
TACCGCGGCAGCTAACGCGTCTGTTAAAGAAGATCCAATCATCGCGGACG10.5747126436781609No Hit
GTACAGTTCCTCTGCGACTACGCGAAAGCGACTGGCACCCACGGATTCGT10.5747126436781609No Hit
GATCTTGGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCTATTCCCCTG10.5747126436781609No Hit
AAGTATGAGTGTGGCCCTCATGAGTTCTAAGGTTTTTACCTTCCTCCTTT10.5747126436781609No Hit
GAATCGATGTTTGCGCATCGCACCGATGCCTCGAAAATCGCGCTGGCAGC10.5747126436781609No Hit
CACTTGCAGCGTGTGACCGAAGATGAGAAGACGCGCCTCGCGCGCGAGCT10.5747126436781609No Hit
TCTTATGCCCGTAGACTAAACCCAAAGAAATTGAGATATACATTATTGCA10.5747126436781609No Hit
GTCAAAGAAAGCCAGATCTTCCTTCAACTGCGCGTTTTCGGTTTCCAGCG10.5747126436781609No Hit
CACCTCCACAAACCAACCTCACCGTCTAGTTTCCCCGGCGGCGCGTCGCG10.5747126436781609No Hit
GTGCAGGCCAGCGGTGAACAGCGGCCCGTACACGCGCGTGGCGAATTGCT10.5747126436781609No Hit
GCATTGCACCGGCGCGCTGCTTCAGAGCTCAGAGGAAGTGCTTCGGCATG10.5747126436781609No Hit
ACCCAATGTCGAGTGGAAGCATCAGCATGTGAGCAGTATGGATATGTAGA10.5747126436781609No Hit
AAATCCCTGTACGTTATGGCTTACAATACTCCTTGTCTACTTTCCTTTTC10.5747126436781609No Hit
CTATACCGCTAAAGATGATTCAACTAGCTGTTCGGTAAAACGCATTCTGC10.5747126436781609No Hit
CCCCTCATCAGTGTTCCACTGGTAGAACCCGAGCTCGTTCGTACGATCGG10.5747126436781609No Hit
GGTTGAGGGGGGAATTCATAGCACGTCTCTCTGCAACGTTTCAGCGCTTT10.5747126436781609No Hit
GAAACATCGTTCCGCCACCTGTATCCTCTTGGTAAGCTGAGCTTTTGTTT10.5747126436781609No Hit
GAGCCAGATAGAAAATAATTTAGGCTTTTTATGCTCTTAGGTCTCTCTTG10.5747126436781609No Hit
CCGGTGGCTTCACCCGTGCTGGTGTTGTTGAGAATCGGATCTGCGCCGGT10.5747126436781609No Hit
GTGTTGAACGTTCCAAAAAAGCCCTCAAGGAAGCTGACTTAGTTCTACTA10.5747126436781609No Hit
CTCATCAACCAGCTCATCCGCTTTAGGATAATCGGCACCGTAATTACCAT10.5747126436781609No Hit
GTCTGGGACATGAATTTCGACAGCGACACTAACGTGATCGACGTCGCCAT10.5747126436781609No Hit
TGGTGAGTAACCTTCTAAATGTTGGGATAATACATTTGGGTGAAAAGTCG10.5747126436781609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)