FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGACGTTGGTCATGACGCCGTCGATGCGGAACCGGAGCGTCATCTGC42.2988505747126435No Hit
GGCCTCTTCGTTCCAATGCGCCTCTGACTGTTGGCCGGGCGGGTCCATCA31.7241379310344827No Hit
AGTCAGATCACCGCCGTGAAGGACCGTCTTAAACCAAGCTGCCTCCGCGG31.7241379310344827No Hit
TTTCTGCTCCGTGTCCATGAAGGACGTTCAGGGCAAGTGCATCCCGAAAA31.7241379310344827No Hit
CACCTCGCTCTCGACCGCAATATCCTTCGGAATAAAGTACGCACCGGTTT31.7241379310344827No Hit
GGCCATTGTTGAACACTTCGGCCCGCAGATGCTGCTGGCCGACGGCAGCC31.7241379310344827No Hit
CCATTACGCGCTGTACGCCGGGCGCTGTGCGGACGCGCGCGATGCCTCGG31.7241379310344827No Hit
CTCTCCTCTACCCCATAAAGAGATGAAGTCCAAGTCTGAAAACTGAATTG31.7241379310344827No Hit
TCTTTGTGATGTTTGCATTCAACTCATAGAGTTGAACATTCCCTTTCATA31.7241379310344827No Hit
CTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTGT31.7241379310344827No Hit
AACCTGCCGCGGCCAATACGTGGCTGGTCGTCAAGGAGGCGAAAGGGTGC21.1494252873563218No Hit
GCGCGAGCTCGAGAACCTGTTGCACCGCGCCCTGGCCATGTCGGGTGGCG21.1494252873563218No Hit
GCTACGATGACGTTGCTAGCGTTGCAAAGGATTACGGTCTGCATATTGTT21.1494252873563218No Hit
CCTAGATCCCCACCTTGGCGCTGGCCGGAGCACCCTGCAGTGAGGCCCCA21.1494252873563218No Hit
AGTCAAATGTGCCCAGCCTACAAATGGGATGCCATGGGACAGTCAATGAT21.1494252873563218No Hit
GACTAAATGAGACAATGTCCTATCAAGCACTTGTCAGAGTCTCTCACATG21.1494252873563218No Hit
TTGCGGTACTGGCAGACCAGCAAGCACCCATGCAGGATTATGCCAGGCTC21.1494252873563218No Hit
GATCTCGACGACCTGCTCATTGGCGCCGAGCTTCATGATCGAGCCCTTGC21.1494252873563218No Hit
GCCGCTGGGTCAGGACGACGGCGCCGAACCCGCGAGCGTGCAGTGCACGC21.1494252873563218No Hit
GCTCAGCAAACAGATGATATAGAGGTTAAGGATGGAGTCGTACAAGTTAT21.1494252873563218No Hit
TGTAATAGCAGCACTTTGGGAGGCCAAGGCAGGTGGATCACTTGAGGTCA21.1494252873563218No Hit
ACTCCACTCTGTGAGATGAACGCACACATCACAATGAGGTTTCTGAAAAT21.1494252873563218No Hit
TATTAGGAATCCACACACCTGCAGAAAACTTGCATTGTGAGGTTCCATGG21.1494252873563218No Hit
CTGGGTGGTGTGCGCGATGAGGCCGAGATCCGCGGTCACCGCCGCACCTA21.1494252873563218No Hit
GTAGGGTGCAGCCTAGTCCCATAACCCAAGCCAGGAGGTGAGGAAGGTTT21.1494252873563218No Hit
GGTGATGGGCGTGGCCATTGTCGGCATGCACTACACGGGCATGTCGGCGG21.1494252873563218No Hit
TCCCGACTGATGGCAACGTTGAGCGCAACATCGAAAAGCTGTCGCAGCCT21.1494252873563218No Hit
CATCAAGGTGGGCGCGGCGACCGAAGTCGAGATGAAAGAGAAGAAGGACC21.1494252873563218No Hit
CAGCAGGCCCGCGCCCATGGCGGTCAACTGCGGGTGCTTTTCGAAGACGT21.1494252873563218No Hit
GGGAGGCCGCCTCTAGCTATAATGGATTTATGAGCAAGGCAAATGTCCTG21.1494252873563218No Hit
GAGATCGGCATTACCGTATCGGCGGGCGTTGCGCCCAACAAGTTCATCGC21.1494252873563218No Hit
CGACAGGACAGCGCCCAGAGCGGCAGCACCGAGCAGGGACCAACGCCGCC21.1494252873563218No Hit
CCAATCAAGCGCGGCGTGAGGAAGACGTTTTCTAGGATCTGGCCCAGGCC21.1494252873563218No Hit
ACATGTATCCAATCTGAATCGGGCAACTACGCACCATGGAACTAACTGAT10.5747126436781609No Hit
TACCGCGATTGAGTATCAGAAAAAAGCCCTGGTTATGATTAAAGATGCTC10.5747126436781609No Hit
GGGGTAGAATGTGCTTCTGGTGAAGATGCTGTGAACATTGTTGAAATGAT10.5747126436781609No Hit
GGGCGCGGTGGCTCACGTCTGTAATCTCAGCACTTTGGGAGGCCGAAGCG10.5747126436781609No Hit
GGTCGTACCGTTGAATACGGTGCGCATATGTTCGGTATGGATGCCACGGT10.5747126436781609No Hit
CAGCAGCGCATGACCGGTGCGGTCAGCAGCGGCACAGGTACGTGCGGCCT10.5747126436781609No Hit
GTGTCGCGCTGCGGCTGGGAAACGCAACTAGGCCCGGGTCGTCACCCGAT10.5747126436781609No Hit
AGAAAACCCCATTATCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACT10.5747126436781609No Hit
ATTATATTCTATCAATTAAAACATATAAATCAAAACAAAACCTAAAGACC10.5747126436781609No Hit
CCTGCGTGCCTTCTTGCGCGTTGTACCAGGCCAGCCATTGGGTCGTGCGT10.5747126436781609No Hit
ACTCTGTGAGATGAATGCACACATCACAAAGAAGGTTCTCCGGATGATGT10.5747126436781609No Hit
GAGAAACTACTTTGTGATGTATACATTCATCTCACAGAGTTGAATGAGCA10.5747126436781609No Hit
CTACAGCATAGTCTAGACCAAGAATTGGCAAAGTATAGTCTGTGGGCTAT10.5747126436781609No Hit
CCGCTTTAGAACTTAGCAGCGATCCAACGAACCGCGCGTTCGAATACACC10.5747126436781609No Hit
TTGCCAGAAGGGCGACTGTTCTGCCGAATCGAGGCGCTCGGCTGCTTCGA10.5747126436781609No Hit
CCTTAGCTGCTGCCCTAGAACCTTCCCTGGGGCCGGGTGTGGTGGCTCAT10.5747126436781609No Hit
ACCCTACCCTACCATAACACCTAGGGACACTAGAATAGCCCCGTTTCCTA10.5747126436781609No Hit
TCTCCACCCTGGTCCAACCTCACCTTGGGCACAGAGCTCCCATTGCAAGG10.5747126436781609No Hit
GTCTTCATCGAGCGCCCGGTCACCACGCTGCTGGTGTCGCATGATCTGGA10.5747126436781609No Hit
GTCCACTGGCCGTATAGACACCTGACAATTCTGGAACCTGCCCAAAGAAA10.5747126436781609No Hit
GTTGAGCTATAACTTATACCCTTGGATTTTCCTTCTTTTTAGGATAGTTC10.5747126436781609No Hit
GCTTGCTGATGATAAAGGGGATCTTTACTCAATCCCAGGAACACCTCCGT10.5747126436781609No Hit
CTATGTGAAAAGACCAAATCTACGTCTGATCGGTGTACCTGAAAGTGATG10.5747126436781609No Hit
TTCAGCTCCTCCGTCGGAATTAATTTGAAAAGCTTATCACTCGGTCTTAT10.5747126436781609No Hit
ACGTACGAGTCGTTGCAGCGCACGGGACACCTGGAGCACTTCGACTGCTT10.5747126436781609No Hit
TGTCACATCCAAGGGCGGCACCACCTATGCCGCGCTGCAGTCGATGGAGG10.5747126436781609No Hit
CTCGATGGGTATGCCCGGAACGGAGATAACCATCAACGGTTCAGCACCCG10.5747126436781609No Hit
ATATTGTGTTTATGACTTCTAATTGTTTACCATTATAAGTAACATCACCC10.5747126436781609No Hit
GTCGCAAGTGGTGCTCGATCTCGTGCAAGAACATCAGGAGAAGCAGACGT10.5747126436781609No Hit
TCGTTACGGACTGCGAATTCCATCGCGGAGATCCGGGTGAAATAGTCGGC10.5747126436781609No Hit
CTCGCGCACCTGCAGCCAGCTCTTGCCGAGCAGCCAGACCAGGCCGGCGC10.5747126436781609No Hit
CTTTGACCACAGCATCATTTCTGAAATGAGACCCGTCGGCTGCAGCGCCA10.5747126436781609No Hit
GGCCAAGTCAGCTGGTAGGGAGATGGTAAACAGCCCATTTCTCTGAGTGC10.5747126436781609No Hit
GTAAAGGAGGCCCTCAAGAGCAGTAGAATCAGCATATCCCGAAGGCAGTG10.5747126436781609No Hit
TCCTTTCTGCTGCTGGAAAACACGACATGTCCGCCACCGAACTCGCTTTC10.5747126436781609No Hit
GAGCTGGGGCCCTTCCTGGCCGACGTCTGCCGCATCACGCGCATGGACCG10.5747126436781609No Hit
CGTCTCCGACGTGCATGACGGCGATGACGCCGCGGTGCAACCGTTCCTCG10.5747126436781609No Hit
CCTCGCTTGGGCGAACCACCGCGTCGCGCCGCGGTGCGGGGGGCGCGCTC10.5747126436781609No Hit
ACTCCGCCCCGTCCGCAACGCACACGCAAACCCAAGAACACCCAGAAAAA10.5747126436781609No Hit
CCAATGATGTCATTGATATTGATGAGACGGACAATACCCATGATTTGGTC10.5747126436781609No Hit
GCAATCGTCGCAGTGAGGGCGATAGGTGAAGATCCCGCTGCGCCGGAAGC10.5747126436781609No Hit
ACGTTCTTCAATCTTATCAATGTTTTGATTTTTAAGGACTGAAATACTGA10.5747126436781609No Hit
GTAATAGAGAAGGTTTAGAAATTTGCCAGTGTTAGAAAAGCCAACTAACA10.5747126436781609No Hit
GCCTTGGACGGTGCGTTTTTGACGCATTGTCACCTCCGCGGATTTTTGGT10.5747126436781609No Hit
GTACGCGCTGATGGCACCGCGGGGCACGCCCGATGCGATCAAGCAGCGCG10.5747126436781609No Hit
CTATGACTACACGCCCGTGGAGCGCGTCGAACAACTGCCGCGCGCCGACG10.5747126436781609No Hit
CCTGATGGCCGAGGCGCTGGGCAGCCAGGGCACCTCGCCGGACGAAGCCG10.5747126436781609No Hit
ACTAGAGTCAGTCCGAGAAAAATCTGTGGCAGGTTACTAATCACTAAGGT10.5747126436781609No Hit
CTCGAACACCTCGGCCCGCTGCCCCTCCGGAATCCCTGGGCCCTGATCGG10.5747126436781609No Hit
GCCGAGACTCTTTGCGGGCTCAGATGATTTTCAATTCTTGGGCCATTGGA10.5747126436781609No Hit
TCTTTGAATTACCTCAACCAGCTATTTTGGATTTCCTGTATGAAAGGTCA10.5747126436781609No Hit
GATTTAGGATCAACCTGCATGAGCACTGACTCATTTGATCTTACCTACAG10.5747126436781609No Hit
GTTTATTTCTAGACGAATAGGTCCCACATTGTTAGGAAAGCTCAGTTTTA10.5747126436781609No Hit
CCTGCAGCGAACATCGTCGAGAGCGTGCGTGTCCAGCGCGCTACGGCGGC10.5747126436781609No Hit
CTAAGATGCAAGATGGGGACTACTGTGAAAATTAAATTAGCAAATGTGTT10.5747126436781609No Hit
CCCATCACGAGTACCAATGACGACACGTCTGTCGGCAAAGTTCTCAATGA10.5747126436781609No Hit
ATTAAGTACTAGACCCTATGCGGTTAGGGTGGTAAGTCGAATTTCAGGTA10.5747126436781609No Hit
ATGCCACAAAGTAGGCATATTAGCTCTCCCTTCAATATTAAGCTTGTTTA10.5747126436781609No Hit
TAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTCCTGA10.5747126436781609No Hit
TTGAAGCACCGTGTGAAATACATCAGGATGATATTGATAAAGAGGTTGAA10.5747126436781609No Hit
AGATAACGTGCGGCGGCTTGCGCGCGTTCCAGTTCGGGCATGTCGGGCAA10.5747126436781609No Hit
CAGGGACTCTTCTGTCTCTCTCAGCTACTCTCCAGCCATCTCCACATCAC10.5747126436781609No Hit
ATACTCATATTTGTGACTTAAGTTCATTATTTTAAATAATTAAGAATTGG10.5747126436781609No Hit
ACTGTGTAGTGTTGGTGAGTTACCACCGGCGAGAGAAACGGGGAGAGCAT10.5747126436781609No Hit
GTACTGATTTTGCTTTGGTTGATATGAAAAGATTGGCACATATTGGTTCA10.5747126436781609No Hit
TTGTTAGAGTTCCTTGCCATCCAGATTCTGGGTTCCGTGTCTGCCATTTC10.5747126436781609No Hit
CCGGAAGATGCAAGCCAAGGTTCGACAAGAAGGGACGCCTTGTCGCGATG10.5747126436781609No Hit
TACCATCACCTAGGGGGTTAGGGTTCAACAGTATAGAGGATGCAGACATC10.5747126436781609No Hit
GTATTCGGCTTTCGGCGTGGCAAGGTTTCCCGGTATCGCGTCATCAGCGA10.5747126436781609No Hit
CCGTAAAGCCATCCCAAGGCTCGGCCCACCATGTGCGGTTGCACCCTCAT10.5747126436781609No Hit
GGTACTTGGGCTTGCGGCCGCAGCGGCGCTGTGGGCCTTCGAGGAAGGGC10.5747126436781609No Hit
ATACAAAAAATTAGCCAGGCATGGTGGTACATGCCTGTAGTCCCAGCTAC10.5747126436781609No Hit
GAAATAATATGGTATTTTATTCAAAAGAACTGATGAAGTACCATGCCTGC10.5747126436781609No Hit
TTATGACACATATCAATTTCTTACCTCAGGCAGTGTACAAAAGCAGGGCT10.5747126436781609No Hit
AGTTGCGGTAACCCTCGCAGCGGCCCGGGTCAAAGTTGATGCTGCGGCCC10.5747126436781609No Hit
GCGGCGATGACGCTGGCGTAGGGGCTGTTGCGAGCCACCAGCGCACTGAA10.5747126436781609No Hit
AAAGAAAACATCTCCTTTCCTAACATCACAGTTAGCGATCTTGCGTTCGG10.5747126436781609No Hit
TCCTTTTGGCCAAGTCCCACCACTGATCTTTACGTTTCCATCGTGGCTGC10.5747126436781609No Hit
CAGATTAAGCGCAATTTTATTGTTCATCTGCACAGCAAAAAGCCCGCAAT10.5747126436781609No Hit
GTATCGCGATGGGCCACGGCGGGATGCTTTATTCCTTACCAAGCCGTGAT10.5747126436781609No Hit
GGCGGCTTCCGCCGCCTCTCGCGTCCCCTCGATCCGCAATTCGCCGGCCT10.5747126436781609No Hit
GGGTTTTATAGGGCAGTCGTACCGCCATGAACTGCGCTTGGTATCCTTCG10.5747126436781609No Hit
GGTTGAGCAGGATCGTCGGGCGCAGGTCTTCGATCAGGCGGCGCTTGATC10.5747126436781609No Hit
TTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT10.5747126436781609No Hit
GATCCCGCTCGACCAGGATGGGCACCTGGCCATAGGGGTTCATGACACTG10.5747126436781609No Hit
GTCTTGGAGGATACCCAGTTAGGAACAATTCACCAAATCAAAACAAAACT10.5747126436781609No Hit
CCTTCACCTCGATCGCCGACGTGGTCGGCAGCTTCAAGTGGGGCTGGCTG10.5747126436781609No Hit
GGGTTGACGCGATCCAGGAGTATGCCGACATCACGGAGCTGGACGCCGCC10.5747126436781609No Hit
CCTCCCGAGTAGCTGGGGCTACAGGTGTGCACCACCACACCTGGTTAATT10.5747126436781609No Hit
AGTCGGAGCGCAGAGGGACACGCAGGTGCGAGAGAGAGCGGCCTAGGAGG10.5747126436781609No Hit
ATACTCATATTTGTGACTTAAGTTCATTATTTTAAATAATTAAGAAGTGG10.5747126436781609No Hit
CTCTTGTTCTTGTCAGAAAACAGCTCTGTCATGTACCGCGCACCAACGGG10.5747126436781609No Hit
ACTTAGCACTATAAAACACAAGCATTTATCTCACACTTTCTGATGGTCAG10.5747126436781609No Hit
CGCATGTGGTGGACAGCCTGGGGAACTTCAGCCAGCAGATCGATGTGGTG10.5747126436781609No Hit
CTTCCGAAACGCGCGTAGGAAATGCGCATGGATCTCAGTATTGATGCCCA10.5747126436781609No Hit
CCATTCTAAGCGCTTCATATTGTTCATTTTTAATCCTCACAACAGCCCTA10.5747126436781609No Hit
CCACCTCTTTGTGCCACGCGGCACCAAAGTGCCCGCAGAAATCGCTGCGG10.5747126436781609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)