FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG33.7037037037037033No Hit
GTGCTCCTGTACACGAGCACGACGATCGGTGGTCTGGCCACCGGTATCTC33.7037037037037033No Hit
AGCCAGGTCGACGTCGCTCTACCAGAGCCGTAGAGACCACGGCAGGCAAC22.4691358024691357No Hit
GCGTTGAAAGCGCCAAGCATGCAGTTGAGCCCGCCCTGGTACACCATGTG22.4691358024691357No Hit
TTACCGAGATGCACGGCTGTCGCGGTAGCGATGACCTGATGACCGTGGGG22.4691358024691357No Hit
GTTCCGATGTGGGTGTTCTTGGTTTCAACAAAGGCTCCGACTTTGGCGTC22.4691358024691357No Hit
CCATTAAAGCCCTCGCTGCTTGGGTCTTATTGGTGTTGACGCGGCCCGAG22.4691358024691357No Hit
CAGGTACACACCGTGGGATCCTTCAAACACGTCTGCGTTCCAGTTCGAGG22.4691358024691357No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT22.4691358024691357No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT22.4691358024691357No Hit
GAAGTGGGAAGGAGACCCAATGGCTAGGCCTGACAAGGTCGCTGCGGTCG22.4691358024691357No Hit
GGGCAAGGTCGTACTTTTTGAGGGCGCCCAGGCCCATCATCTTGACGTTG22.4691358024691357No Hit
GGTTTCTTGGGGCTGCCGCGGGGGCTGCTGGTGCATTCGCGCTTGCCGGC22.4691358024691357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC22.4691358024691357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGTGAAAGTCCAGATTAAGCTCATCGGCCAAGATGTCGATGTCCTCA11.2345679012345678No Hit
CTGTACGTAAGCGTGCTCAGCGCCGAACAGAGCGCAGGCATGCTCGGCGG11.2345679012345678No Hit
GGTAGTGACGGAGGACGCGGCGGCGGCGTTGGCCCTGACGCAGCGTTTCG11.2345679012345678No Hit
GAATGTTGCACTCGGTGAGTTGAATGCACACATCACAAAGTAGTTTCTGA11.2345679012345678No Hit
GGTTGAACGAGCTCACAATCACCTGACGTCCAGGCTTGAGTCTTTCAAGC11.2345679012345678No Hit
ATATGTCATGGTCCGCAGCTGTTATTGACAGCCAAGACACTTGAAGGCAG11.2345679012345678No Hit
GGCTGGACCTGCACGAACCCCAGGATCCCAAGCTTCCGCAGCCCGCGGCC11.2345679012345678No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGC11.2345679012345678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.2345679012345678Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTCATGTCAGCGCATGGCCTTGGGTATGCCTCGTCGCTGGGGCATTGA11.2345679012345678No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG11.2345679012345678No Hit
GTATCTGAGACTTACAGTTTTATTCTTTTTTCCCAAATTCAGGAAATTTT11.2345679012345678No Hit
TTGATTTTGTATCCTGAGACTTTGCTGAAGTTGCTTATCAGGTTAAGGAG11.2345679012345678No Hit
ATCCTCGGCCACGCCCCCATCATCCTGCCCGCCGTGTTGCAGGTCCGGCT11.2345679012345678No Hit
AAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCG11.2345679012345678No Hit
TCTAGCGACCTGTCCGAGTCGAACACTAGAATTTGGAATATGACCTCACA11.2345679012345678No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT11.2345679012345678No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA11.2345679012345678No Hit
GTGCACTGGGCAGCAGGGAATGCCAAATCGGCCCCGGGGAGCTGCGGAGT11.2345679012345678No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGGTATC11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
TTTCAGTGCCCTCCTCAAGGTCAGATGTTTTCACTGGTAAAGTCAGGAAA11.2345679012345678No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC11.2345679012345678No Hit
CCCCGGTCCCCTTCGATGATCTTGCCCTCACTGTCGACGACGACCATGTC11.2345679012345678No Hit
TGGCAGCCCTCGGCCCACTGCTGGGCGGAGTCTTGACTGAATACGCGTCA11.2345679012345678No Hit
CTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAG11.2345679012345678No Hit
GTCCCAGGCTGTGTTCGTGCCACACCAGGCGGGCCGCCAGCACCAGGACC11.2345679012345678No Hit
TCCCAAAGCTCCCCGCAGTCTCCTTCCTTCTTGGGGGGGTCTGGTTTAAC11.2345679012345678No Hit
GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
TTCGTGAACTGGGTCGCGCGACGCAAGGCGTCACGCTGATCGGGCTCGAC11.2345679012345678No Hit
CATTAGAACTTCATGAAGTTTTAACTGTTGATTCTTTCCCAAGCATCATC11.2345679012345678No Hit
ATGAAGAAGCTTCATCTCAACCTCCGTCATGTTTTAGAAACCTTTTATCT11.2345679012345678No Hit
GAAGTGCAGTTTTCTTTTGGCAGTTCAGAATCCAAGCACTTGATTTGCTG11.2345679012345678No Hit
CAACATTACTGAAGGCTGGGTCATCGCGGTCGGAATGATTGCTCAGCAGC11.2345679012345678No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
TTATCGATGAGTCGGAAGTGCTTAGCCCGGCTGACTACGCCAATCACATC11.2345679012345678No Hit
GGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCT11.2345679012345678No Hit
GCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGAAGTG11.2345679012345678Illumina Single End PCR Primer 1 (95% over 22bp)
TTACAACGGAAGTAAAATCTGTCGAAATGCACCATGAAGCTTTGAGTGAA11.2345679012345678No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTCGCCCGCTGTCGCTTCTCCAGCCACCCTGCCAATCTCGAATACCG11.2345679012345678No Hit
GTCGTGGGGGGTACCACGACCTGAGCAGATGCTGCGAACTTCACCAAGGA11.2345679012345678No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCTTGAATCTGCGTTG11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
GGATCATGCAATATTTGTCATTCTGTGTCTGACTTTTTTAACTTAGCATA11.2345679012345678No Hit
GTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCG11.2345679012345678No Hit
CCCTGCGAGAAGGCCGCACTGGGCCTGGTGAGGCACCTGCAGATCCTGGG11.2345679012345678No Hit
CGCCCACACCTATGGATGGGTTCCTGCCGTCGTTGGCACCTCAGAGGAAG11.2345679012345678No Hit
GTCTTTGAAGAGAGCCAAGAGGGCTGGCGGGATAACACCGATGATGAAGC11.2345679012345678No Hit
CTCCTCATCTCTCTCCCCTGCCTTTGCACCAAACTTCTATGTAATTCTAC11.2345679012345678No Hit
TGCTCGACCTGTCAGTCTCACAGTCAAGCCCCCTTGTGCACTTACACTCA11.2345679012345678No Hit
GATAAGAAGGATGATATTGACGACGAAGATGGCGACGAAAAGAACGATGC11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)