FastQCFastQC Report
Tue 10 May 2022
EGAF00003636599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333450
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT8790.2636077372919478No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC7390.22162243214874797No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC7040.211126105862948No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC6970.20902684060578797No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT6820.20452841505473085No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA6210.18623481781376516No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT5970.17903733693207377No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT5590.16764132553606237No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT4960.14874793822162244No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA4800.14394961763382816No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA4510.13525266156845106No Hit
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG4230.12685560053981107No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG4200.12595591542959966No Hit
GCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAA3950.11845853951117108No Hit
TATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT3910.11725895936422251No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATG3830.1148597990703254No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA3820.11455990403358823No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG3820.11455990403358823No Hit
CTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT3720.11156095366621684No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC3700.11096116359274255No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG3650.10946168840905682No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC3560.10676263307842254No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC3550.1064627380416854No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA3500.1049632628579997No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA3420.10256410256410256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)