FastQCFastQC Report
Tue 10 May 2022
EGAF00003636600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333450
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT9120.27350427350427353No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC7830.2348178137651822No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC7410.2222222222222222No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT6770.20302893987104512No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC6460.19373219373219375No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA6270.18803418803418803No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT6060.18173639226270805No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT5930.1778377567851252No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT5260.1577447893237367No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA5180.15534562902983956No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA5070.15204678362573099No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG4270.12805518068675964No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA4240.1271554955765482No Hit
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG4210.12625581046633677No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG4170.1250562303193882No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG4150.12445644024591393No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA4050.12145748987854252No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC4020.12055780476833107No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATG3990.11965811965811966No Hit
GAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGG3950.11845853951117108No Hit
TATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT3800.11396011396011395No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC3770.11306042884990253No Hit
CTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT3760.1127605338131654No Hit
GCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAA3700.11096116359274255No Hit
GAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGG3500.1049632628579997No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA3470.10406357774778827No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC3380.10136452241715399No Hit
ACTTTAAGCTGTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTT3380.10136452241715399No Hit
GTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCC3340.10016494227020542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)