FastQCFastQC Report
Tue 10 May 2022
EGAF00003636610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354347
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75440.5570212065297889No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG72020.5317691847067259No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG56420.4165845237594206No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC55420.4092008916474138No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA52950.3909633203307572No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG48330.35685093997328604No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT47170.34828592672335823No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC46160.3408284582902314No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC44330.32731641152525903No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40650.3001446453530742Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC38640.2853035448079406No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC34470.25451379890087256No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT34220.25266789087287084No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA31030.22911410443556932No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG30470.22497927045284555No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA29350.21670960248739798No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA25910.1913099080220948No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC25290.1867320561126506No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC25270.18658438347041048No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC23450.1731461730265582No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA23110.1706357381084759No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22610.16694392205247252Clontech Universal Primer Mix Long (96% over 26bp)
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT22560.16657474044687218No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22190.1638427965654297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC21720.1603724894727865No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG20620.15225049414957909No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA20050.14804182384573525No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT19980.14752496959789477No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG19310.14257793608285024No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT19170.1415442275871693No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT19100.14102737333932885No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT18960.1399936648436479No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG18920.13969831955916762No Hit
GAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACAC18910.13962448323804755No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC18900.1395506469169275No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG18450.13622801246652444No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT17900.13216701480492074No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG17820.13157632423596022No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG16800.12404501948171333No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA16500.1218299298481113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.11946716757226913No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15950.11776893218650761Clontech Universal Primer Mix Long (96% over 26bp)
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT15690.11584918783738585No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC15580.11503698830506509No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC15070.11127133592794165No Hit
GAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACAC14910.11008995479002057No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC14860.10972077318442024No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT14530.10728417458745801No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT14500.1070626656240978No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA14380.10617662977065702No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14340.10588128448617673Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA13970.10314934060473423No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG13660.10086041465001215No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGG13610.10049123304441179No Hit
CACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGAC13590.10034356040217168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)