FastQCFastQC Report
Tue 10 May 2022
EGAF00003636622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543399
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52830.3422964508853511Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50710.3285605342494067No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26900.17429064033344585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24090.15608407158485912Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT23510.15232613212785548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.13684082988261623No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18650.1208371911605489Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17700.1146819454982153Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA17020.11027608544517652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)