FastQCFastQC Report
Tue 10 May 2022
EGAF00003636628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445089
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27790.6243695081208477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17720.3981226226664779Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT10730.24107538042953208No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG9640.21658589630388528Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT7690.17277443387726948No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG7490.16828095055146275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7460.16760692805259172Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.14446548892468697No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6120.13750058976968652Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5970.13413047727533145Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG5440.12222274646194356No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5400.12132404979678221No Hit
GGACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGG5360.12042535313162087No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA5140.11548252147323346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.10649555482161994No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4620.1037994648261359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC4610.10357479065984557No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4530.10177739732952285Clontech Universal Primer Mix Long (96% over 26bp)
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG4480.10065402649807116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)