FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003636632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586113
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74010.4666124040342649Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT47940.3022483265694184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41090.2590609874580185No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39970.2519996998952786Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38770.2444340346494859Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA37960.23932721060857579No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG33750.21278433503791974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27600.17401030065323217Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.15900506458240996No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24780.1562309873256193Clontech Universal Primer Mix Long (96% over 26bp)
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA22560.1422345066209028No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC21670.13662330489693988No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16380.10327133060507039Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT16040.10112772545209578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)