FastQCFastQC Report
Tue 10 May 2022
EGAF00003636721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.8034466697717745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCTCGTATGCCG550.6404285048905449No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT540.6287843502561714Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCTCGTATG510.5938518863530508No Hit
ACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCTCGTATGCCGTC430.5006986492780624No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT300.3493246390312063Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG300.3493246390312063Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG260.30274802049371213Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCGCGTATGCCGTC210.24452724732184444No Hit
ATACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCGCGTATGCCG190.22123893805309736No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170.19795062878435027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACATCTCCGAGCCCACGAGACTGTAGATAGATAATCTCGTATGCCGTCT170.19795062878435027TruSeq Adapter, Index 7 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG160.18630647414997673Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140.16301816488122964Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC140.16301816488122964Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTAGATAGATAATCGCGTATG120.13972985561248255No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG110.12808570097810898No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA110.12808570097810898No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.12808570097810898Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACTCTGGGCAGGATCCAACGTCGCTCCAGCTGCTCTTGACG100.11644154634373545No Hit
GCGGTATGCCGCCAAACGCTTCCGCAAAGCTCAGTGTCCCATTGTGGAGC100.11644154634373545No Hit
GTCTTTAGCGGCCTGTCATATTAACAACCGTTGCGACGGGTCCGAGACAT100.11644154634373545No Hit
TCTCTACACCGTTCCCATCCGGGAACAGGGCAACATCTACAAGCCCAACA100.11644154634373545No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90.10479739170936189Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCGTAGAGAGAGTGGAGCTGCTTTATTGCCCTTGGAGCCCGCGCTCTCG90.10479739170936189No Hit
CCACAGGAATAACCACCTTCCTCTTCTTCTGTCACCTGGTCTCCTGAATT90.10479739170936189No Hit
TCTCTAGCCTATTCCTTACCACCTGTAATCTTGACCATATACCTTGGAGT90.10479739170936189No Hit
TTTTTTCGTCAAGCTGTTCTTTATTTCAGGGAGAGGGCAGGGGAGGGGCT90.10479739170936189No Hit
GAATTTATTGGCTGTCTTTGAGTGTCTTTGGTATGGCTTTGGCAGGGCTG90.10479739170936189No Hit
ACATGGGACTCTGGGCAGGATCCAACGTCGCTCCAGCTGCTCTTGACGAC90.10479739170936189No Hit
CATCTCCGAGCCCACGAGACTGTAGATAGATAATCTCGTATGCCGTCTTC90.10479739170936189RNA PCR Primer, Index 7 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)