FastQCFastQC Report
Tue 10 May 2022
EGAF00003636722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2683.12063344201211No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA720.8383791336748951Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT420.4890544946436889Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG370.4308337214718212Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT240.2794597112249651Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.2328830926874709Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG180.20959478341872378Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG180.20959478341872378Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT130.15137401024685607Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG120.13972985561248255Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT110.12808570097810898No Hit
CAGGTTTATTGGGCAGCAGCTGGGAAAATCAGCGGTTGGACTTGGCCACA100.11644154634373545No Hit
GTACATGGGAGTTCCTGGCGCGAGCGGCCGCGGGCCAGTTTCTATCTCCT100.11644154634373545No Hit
CTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCC100.11644154634373545No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC90.10479739170936189Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90.10479739170936189Clontech SMART CDS Primer II A (96% over 26bp)
ACGCTACCCTATGGCCGTGGGCCTCAACAAGGGCCACAAAGTGACCAAGA90.10479739170936189No Hit
CTTCGGTAATACAGAGGGGGGAAGGTGTACTCAATTCCCAGCCCCCAGCA90.10479739170936189No Hit
GCCACGCAGAACAAGACAGGGAAGTATGTGCCGCCGAGCCTGCGCGACGG90.10479739170936189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)