FastQCFastQC Report
Tue 10 May 2022
EGAF00003636734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849342
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50800.5981100663807983No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36960.43516039475264384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19610.23088461420723339Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19400.2284121119643206Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12530.14752596716046068Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT12520.1474082289584172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.1404616750378528No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11870.13975524582559204Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG8590.10113711555533578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)