FastQCFastQC Report
Tue 10 May 2022
EGAF00003636755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393861
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT181580.7585235734238538No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA108220.45207303180928216No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG102960.4301001603685427No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG97070.4054955571772964No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG96990.4051613690185019No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC91250.38118336862499536No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG84740.35398880720309156No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT82070.3428352774033246No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG80920.33803132262065344No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA72740.30386058338391414No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG72490.30281624538768126No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG70960.29642489685073614No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG66310.27700021012080484No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG63070.2634655896896269No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT58950.2462548995117093No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC58170.24299656496346278No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC57990.24224464160617515No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT57100.23852679833958612No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC56520.23610393418832587No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT56220.23485072859284645No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT55710.2327202790805314No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT53760.22457444270991508No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT49810.20807390236943585No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG48650.20322817406691532No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA48090.20088885695535372No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG47980.20042934823701125No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG47010.19637731681162773No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG46830.19562539345434007No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC46450.1940379997000661No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45440.18981887419528537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA44080.18413767549577859No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA42670.17824760919702523No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC41890.17498927464877867No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG41560.1736107484937513No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT41000.1712714313821897No Hit
CAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC39430.1647129887658473No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG38350.16020144862212135No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA36270.1515125564934639No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG35940.15013403033843653No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC35380.14779471322687493No Hit
GTTCTGGAGATGAGCATTGGGGCGCTGGAAGCCCAGCAACGCCTGGCCCT34610.1445781521984777No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT34530.14424396403968318No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCC34360.1435338142022448No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC33410.13956532981655995No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGT32640.1363487687881627No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT32430.1354715248713271No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC31780.13275624608112166No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAA31600.132004322723834No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTATTGAGAG31570.13187900216428608No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG30120.12582184178613545No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAGGA30080.12565474770673818No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC30000.12532055954794366No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG29880.12481927730975191No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA29610.12369139227382041No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA29140.12172803684090262No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC28440.11880389045145061No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG28280.11813551413386157No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT27970.11684053501853282No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC27930.11667344093913556No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC27610.11533668830395749No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC27530.11500250014516299No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA27410.11450121790697121No Hit
CTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT27350.11425057678787533No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA26780.1118694861564644No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26600.11111756279917674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC26220.10953016904490277No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT25990.10856937808836854No Hit
CCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGC25960.10844405752882061No Hit
CTGCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGG25420.10618828745695762No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTGCCCAC24080.10059063579714947No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG24050.10046531523760151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)