FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003636778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072700
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG245111.1825638056641097No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG225021.085637091716119No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG214771.0361846866406137No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC212031.0229652144545762No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA167260.8069667583345396No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA130870.6313986587542819No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC129890.6266705263665749No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC117740.5680513340087808No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT117040.5646740965889901No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG107780.5199980701500458No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC106630.5144497515318185No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT106560.5141120277898393No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT98740.4763834611858928No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC94530.45607179041829493No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC93770.45240507550537945No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC92940.44840063685048487No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA91890.44333478072079896No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT89770.4331065759637188No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG86240.4160756501182033No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG82400.3975490905582091No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA81990.39557099435518883No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG74710.3604477251893665No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA74480.359338061465721No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT70850.34182467313166404No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA70580.34052202441260193No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT69870.3370965407439572No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG69740.3364693395088532No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT69020.33299560959135427No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT68450.33024557340666766No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC66560.321127032373233No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA65990.31837699618854637No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC64630.3118155063443817No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC63600.3068461427124041No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT62650.3022627490712597No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC61330.2958942442225117No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG59830.28865730689438895No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG57170.2758238046991846No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT55990.27013074733439474No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC55910.26974477734356156No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG55770.2690693298596034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54200.2614946687895016No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT52300.2523278815072128No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA52130.25150769527669226No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC51340.24769624161721424No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG51250.2472620253775269No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC50030.2413759830173204No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT49980.24113475177304963No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG49980.24113475177304963No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC49280.237757514353259No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA48940.23611714189221786No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA47390.22863897331982436No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG46890.22622666087711682No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC46430.22400733342982584No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC45810.22101606600086846No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC44610.21522651613837024No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT44040.2124764799536836No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG43960.2120905099628504No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC43690.21078786124378832No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT43210.20847204129878905No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG43180.2083273025522266No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA41330.19940174651420853No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA40060.19327447290973127No Hit
CACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGAC39650.19129637670671104No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC39000.18816037053119122No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT38990.18811212428233706No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG38760.18700246055869155No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC37860.18266029816181792No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC37520.18101992570077677No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC37520.18101992570077677No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA37000.17851112076036088No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG35980.17359000337723743No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34730.16755922227046846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTC34550.16669078979109375No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC34300.16548463356973994No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT33920.16365127611328217No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC33750.1628310898827616No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT33680.16249336614078255No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA33600.16210739614994932No Hit
CATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG33140.15988806870265837No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG32990.1591643749698461No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC32780.15815120374390892No Hit
AGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCC31860.15371254884932695No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC31840.15361605635161865No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG31810.1534713176050562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.15294060886766053No Hit
GAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACAC31480.1518791913928692No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC30730.14826072272880783No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC30540.14734404400057896No Hit
TTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTC30240.1458966565349544No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG29750.14353259034110097No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT28930.13957639793506055No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC28890.13938341293964393No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC28870.13928692044193564No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG28740.13865971920683165No Hit
GAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACAC28700.13846673421141506No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT28410.13706759299464466No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT28250.13629565301297825No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT28090.1355237130313118No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGG28040.13528248178704105No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC27890.13455878805422877No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA27430.13233946060693783No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT27310.131760505620688No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC27010.13031311815506344No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA26920.1298789019153761No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT26110.1259709557581898No Hit
TTTGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC25960.12524726202537753No Hit
AAACTCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT25930.12510252327881508No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC25880.12486129203454431No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGAGTTTCGATGACACGCTGA25560.12331741207121147No Hit
GGAGAGTTTCGATGACACGCTGAAAGCTCGGGCAGATCTCTGCAGAAGCG25030.12076036088194143No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT24610.11873401843006708No Hit
AGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTT24060.11608047474308872No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC23940.11550151975683891No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA23870.11516379601485985No Hit
GTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAG23840.11501905726829739No Hit
GACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCC23650.11410237854006852No Hit
TAACTGGAGGGTGTGTCCATGAGGAGAGTTTCGATGACACGCTGAAAGCT23110.11149708110194433No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG23070.11130409610652771No Hit
ACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA22970.11082163361798619No Hit
GTATCAACGCAGAGTACATGGGACTAGCCCACCAGACTCAGAGACGGAAC22790.10995320113861148No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC22780.10990495488975732No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG22690.10947073865006995No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGA22640.1092295074057992No Hit
CTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAG22590.10898827616152844No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT22530.10869879866840353No Hit
TTTTCGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG21620.10430839002267572No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG21580.10411540502725913No Hit
ACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCC21520.10382592753413424No Hit
GAGTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC21510.10377768128528007No Hit
CCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGG21190.10223380132194722No Hit
CTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCAT21140.10199257007767647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)