FastQCFastQC Report
Wed 11 May 2022
EGAF00003636824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5073856
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT225840.4451052611662609No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG164330.32387596336987096No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG148410.2924994323843641No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG147900.29149427969575803No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT143380.2825858676320337No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA141450.2787820545163284No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT132590.26131999016132895No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC124100.24458715422747512No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG123330.24306957075644242No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG109300.21541801738165214No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG103850.2046766798269403No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG96410.1900132758990401No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG94710.18666276693701989No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT91990.18130195259778756No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA90270.17791202588327298No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC89390.17617764477352135No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG89230.1758623027535665No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG88650.1747191879312302No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT85100.16772253686348212No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG83970.16549543384755105No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG78300.1543205010154013No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA76880.1515218405883021No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT73300.14446606289181246No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC72320.14253459301958904No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG70420.1387899065326253No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT70040.13804096923523254No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA68250.13451308038698773No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC67640.1333108389359099No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC67180.1324042306285397No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC66620.13130053355869775No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC65220.12854129088409288No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG64000.12613680798193722No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT60750.11973142320160444No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA56830.11200554371271079No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA56730.11180845495023903No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA56290.11094126439536321No Hit
CAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC56140.11064563125165554No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG55600.10958135193430796No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT55420.10922659216185875No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA53390.10522569028368167No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT53090.10463442399626635No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG53020.1044964618625361No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTATTGAGAG52440.1033533470401998No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC52300.1030774227727393No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG52250.10297887839150342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)