FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003637079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2166.631869818851705No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2116.478354313785692No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1404.298434141848326No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG962.947497697267424No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG912.7939821922014123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC662.026404666871354No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC651.9957015658581518No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG441.3509364445809027No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG441.3509364445809027No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATG411.2588271415412957No Hit
ATACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATGCCG381.1667178385016888No Hit
ATACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCGCGTATGCCG371.1360147374884864No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA371.1360147374884864No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT341.0439054344488794No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC320.9824992324224747No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT280.8596868283696654No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCGCGTATG280.8596868283696654No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG270.8289837273564631No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC260.7982806263432606No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA260.7982806263432606No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT260.7982806263432606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG220.6754682222904513No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA220.6754682222904513No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA220.6754682222904513No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC200.6140620202640467No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC200.6140620202640467No Hit
ACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCGCGTATGCCGTC180.552655818237642No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA170.5219527172244397No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT170.5219527172244397No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG160.49124961621123736No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT150.46054651519803497No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC150.46054651519803497No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC150.46054651519803497No Hit
ACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATGCCGTC150.46054651519803497No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140.4298434141848327No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG140.4298434141848327No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG130.3991403131716303No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC130.3991403131716303No Hit
CTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG120.368437212158428No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA120.368437212158428No Hit
CACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCGCGTATGCCGTCT110.33773411114522567No Hit
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110.33773411114522567No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100.30703101013202333No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA100.30703101013202333No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG100.30703101013202333No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC90.276327909118821No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT90.276327909118821No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA90.276327909118821No Hit
CACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATGCCGTCT80.24562480810561868No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT80.24562480810561868No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80.24562480810561868No Hit
CATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATGCCGTCTTC80.24562480810561868RNA PCR Primer, Index 41 (95% over 22bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC60.184218606079214No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC60.184218606079214No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA60.184218606079214No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA60.184218606079214No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG60.184218606079214No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA60.184218606079214No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG60.184218606079214No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG60.184218606079214No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA50.15351550506601166No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA50.15351550506601166No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA50.15351550506601166No Hit
ACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAA50.15351550506601166No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC50.15351550506601166No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT40.12281240405280934No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG40.12281240405280934No Hit
TATACACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCGCGTATGCC40.12281240405280934No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.12281240405280934No Hit
GCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG40.12281240405280934No Hit
CCACAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.12281240405280934No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.12281240405280934No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.12281240405280934No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA40.12281240405280934No Hit
ACATCTCCGAGCCCACGAGACTTTCAACTCGCCATCTCGTATGCCGTCTT40.12281240405280934RNA PCR Primer, Index 20 (95% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT40.12281240405280934No Hit
GATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.12281240405280934No Hit
CTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.12281240405280934No Hit
CCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.12281240405280934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT352.52237E-453.7142871
ACTTTTT352.52237E-453.7142873
TACTTTT352.52237E-453.7142872
CTTTTTT458.718234E-441.777784
TGTACTC3751.2307364E-411.289
CCCATGT3952.002267E-410.708865
ATGTACT4002.2520337E-410.5758
CATGTAC4002.2520337E-410.5757
TCCCATG3650.001420836510.301374
CTTCCCA3650.001420836510.301372
CCATGTA4153.174872E-410.1927716
TTCCCAT3700.001592668810.1621623
GCTTCCC3700.001592668810.1621621
Produced by FastQC (version 0.11.5)