FastQCFastQC Report
Tue 10 May 2022
EGAF00003637323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317220
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3269310.306096715213418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT183795.793770884559612Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG120713.8052455708971693Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG106873.368955299161465Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG72922.298720131139272Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC58251.836265052644852Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53301.6802219279994954Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49131.5487674169346195Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT46061.4519891557909337Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC42971.3545804173759537Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT26700.8416871571779838Clontech Universal Primer Mix Long (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG26440.8334909526511568Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG24880.7843137254901961Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG24500.7723346573356031Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22510.7096021688418133Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC22320.7036126347645167Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21610.6812306916335666Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21550.6793392598196835Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21340.6727192484710925Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20390.64277157808461Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19440.6128239076981276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC19370.6106172372485972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC18720.5901267259315301Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC17150.5406342601349221Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16690.5261332828951516No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG14260.44953029443288567Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.44953029443288567No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT13260.4180064308681672Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC12880.40602736271357415Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTCTATGTAACATCTCGTATG12650.3987768740936889No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11350.3577958514595549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10100.31839102200365677Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA9750.3073576697560053Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9540.3007376584074144Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8830.2783557152764643No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8590.27078998802093185Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8540.2692137948426959Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT8200.25849568123069167Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8200.25849568123069167Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT7830.2468318517117458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7760.2446251812622155Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.24336422671962674No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7570.238635647184919Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA7000.22066704495302944Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.20395939726372864No Hit
ATACACATCTCCGAGCCCACGAGACACTCTATGTAACATCTCGTATGCCG6470.20395939726372864No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT6440.20301368135678707Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC6260.19733938591513775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA6260.19733938591513775Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT6090.19198032910913562Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTCTATGTAACATCGCGTATG5990.18882794275266376No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5840.184099363217956Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG5830.1837841245823088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT5780.18220793140407288Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.17306601097030452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5290.1667612382573608Clontech Universal Primer Mix Long (95% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5150.1623478973583002Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA5090.16045646554441714Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC5090.16045646554441714Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5030.15856503373053402Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4900.1544669314671206Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG4590.14469453376205788Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4370.13775928377781982Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4330.13649832923523106No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4290.13523737469264233Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4220.13303070424311203Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA4050.12767164743710988Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG4020.12672593153016834Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3980.1254649769875796Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3930.12388878380934368No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3740.11789924973204716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3730.11758401109639997No Hit
ACACATCTCCGAGCCCACGAGACACTCTATGTAACATCTCGTATGCCGTC3640.11474686337557533No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT3600.11348590883298658Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTCTATGTAACATCGCGTATGCCG3590.11317067019733938No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.11222495429039783No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3410.10749637475569007Clontech SMART CDS Primer II A (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3400.10718113612004287Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3370.10623542021310133Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG3250.1024525565853351Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3180.10024588613580479Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)