FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003637788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2622.22222222222222No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA65.128205128205128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG43.418803418803419Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43.418803418803419Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG32.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCGTGGTGGCAGGCGCCTGTAGTCCCAGCTACTCGGGAGGCT21.7094017094017095No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTTAAGAACTGCTTGGCAGCATTTTGCAGAGATCATGAATATACAGGGCA10.8547008547008548No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACACTGCGTTTTAAC10.8547008547008548No Hit
GGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCATGGGAAGCAGTGGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.8547008547008548No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.8547008547008548Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.8547008547008548Clontech SMART CDS Primer II A (96% over 26bp)
GCGCTGGGCGCGCGTATCCGCCGGGAGCGTTGACGGTGGCGTTCCGGGGG10.8547008547008548No Hit
ACATGGGAGGAGGCTGAGGCATGAGAATCCCTTGAACCCGAGAGGTGGAG10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8547008547008548No Hit
ACCTTGACCTTCTGGGCTTAAGCGATCTTCCCACGTCAGCCTCCCACGTA10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTTTTAACCA10.8547008547008548No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATGGGGCAGT10.8547008547008548No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG10.8547008547008548No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTG10.8547008547008548No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAG10.8547008547008548Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAG10.8547008547008548No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTATGC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ATACGACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTCTCTT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGCTGTCTCT10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCGATGCCCTGGGCGGCCAGACGCTTTTCCAACGTGCCGCGCGCCTTGAG10.8547008547008548No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA10.8547008547008548No Hit
GGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAC10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAT10.8547008547008548No Hit
ACTCTGCGTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8547008547008548Clontech Universal Primer Mix Long (95% over 23bp)
CATTCCATTCGAGTCCATTCCATTCAATGCCATTCCATTTGATTCTATTC10.8547008547008548No Hit
CTCTGGTGGAGCTATATAAGCCGCTGTTGCGTAAATCAACGGCATGATCC10.8547008547008548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8547008547008548No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAGCTGGGTTG10.8547008547008548No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAACAAAGCA10.8547008547008548No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTA10.8547008547008548No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA10.8547008547008548Clontech SMART CDS Primer II A (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAACAGTGGC10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCAG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCATTGCTTCCCATGTACTCTGGTTGATACCACTGCTTCCCATGTACT10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.8547008547008548No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACTGTCTCTTATAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAAA10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGCTG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph