FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003637811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12011.049723756906078Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT857.826887661141805Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC454.143646408839778Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA343.1307550644567224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG232.117863720073665Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC211.9337016574585635Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG171.5653775322283612Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC141.289134438305709Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131.1970534069981584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC131.1970534069981584Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC121.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC111.0128913443830572Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA111.0128913443830572Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA111.0128913443830572Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC111.0128913443830572Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG111.0128913443830572Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA100.9208103130755065Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA90.8287292817679558Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.7366482504604052Clontech Universal Primer Mix Long (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGAATCGGGTATCTCGTATGCCG80.7366482504604052No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70.6445672191528545Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT70.6445672191528545Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.6445672191528545Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60.5524861878453038Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT50.46040515653775327Clontech SMART CDS Primer II A (96% over 26bp)
GTCTCTATCTTGCACGGCCACCTGCAGTGGTCCTATAGCTGCATTGGTCT50.46040515653775327No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.46040515653775327Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.46040515653775327Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG50.46040515653775327Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAAGAATCGGGTATCTCGTATGCCGTCT50.46040515653775327No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT40.3683241252302026Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG40.3683241252302026Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG40.3683241252302026Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAAGAATCGGGTATCGCGTATGCCG30.2762430939226519No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC30.2762430939226519Clontech Universal Primer Mix Long (96% over 25bp)
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA30.2762430939226519No Hit
CCACCGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.2762430939226519Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.2762430939226519Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30.2762430939226519Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC30.2762430939226519Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG30.2762430939226519Clontech SMART CDS Primer II A (96% over 26bp)
TGCCTCTCCTGCTGGCTGTCTCCGTCCTGTCCAGGCCCAGGCCCAGAGCG30.2762430939226519No Hit
GGGCTACCGCATCGAGCAGGCGCGGCTGGAGCGCGACGCCCAGGAACGCA30.2762430939226519No Hit
CTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.2762430939226519Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.2762430939226519Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30.2762430939226519Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT30.2762430939226519Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.2762430939226519Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2762430939226519Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGGGGTCCCTCCCTTGGCCTGGCTTC30.2762430939226519No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.2762430939226519Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTGCGTTGATACCACACCCATGTACTCTGCGTTGATACCACTGCTTC20.1841620626151013Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT20.1841620626151013No Hit
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.1841620626151013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.1841620626151013Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
ACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCT20.1841620626151013Clontech SMART CDS Primer II A (96% over 25bp)
CATCTCCGAGCCCACGAGACTAAGAATCGGGTATCTCGTATGCCGTCTTC20.1841620626151013Illumina PCR Primer Index 2 (95% over 21bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC20.1841620626151013No Hit
GCGGTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.1841620626151013Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA20.1841620626151013No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC20.1841620626151013No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG20.1841620626151013Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.1841620626151013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGATACCACTGCT20.1841620626151013Clontech SMART CDS Primer II A (96% over 25bp)
AAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTATGCGTTGAT20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
TGCCACTGGCGTGACAACCGATACACCAGAGGTGTGTCCACTCCGGTCCT20.1841620626151013No Hit
TGGCTGCATAGCATACCTAAGCGCAAGGGTGGTGAAGCCGACAGAGGGCG20.1841620626151013No Hit
GCGTTGATACCACTACTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.1841620626151013Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTACTCTGCGTTGAT20.1841620626151013Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCCCCATGTACTCTGCGTTGATAC20.1841620626151013Clontech SMART CDS Primer II A (95% over 24bp)
GGTTGAGGGTGGAGGTGGGGGACACAGGTGCGCAGCTGCACAGAGTCAGC20.1841620626151013No Hit
TACCACAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGAATCGGGTATCTCGTATG20.1841620626151013No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.1841620626151013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20.1841620626151013No Hit
ATCCTCTATTGGTACGGAATCTCACTGTTCTCGGTGCTGGTTGCGCTGAT20.1841620626151013No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1841620626151013Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)