FastQCFastQC Report
Tue 10 May 2022
EGAF00003637849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415905
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA380689.153051778651374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG143393.4476623267332682Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG122032.9340835046464937Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80191.9280845385364447Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC73091.757372476887751Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT64061.540255587213426Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59161.4224402207234825Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGT47921.1521861963669588Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCT47731.1476178454214303Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT47431.1404046597179645Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG35770.8600521753765885Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA35190.8461066830165542Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34790.8364891020785997Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG33470.8047510849833496Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT33200.7982592178502301Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30010.7215590098700425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG29630.7124223079789856Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25330.6090333128959738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19140.46020124788112665Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA19120.45972036883422895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18450.44361092076315506Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18280.4395234488645244No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC17950.43158894459071184Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG17790.42774191221553Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGCTCCCACTGCTTCCCATGTACTCTGCGTTGCT17450.4195669684182686No Hit
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCC16700.4015340041596037Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC15920.38277972133059235Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGC15560.3741238984864332Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15480.3722003822988423Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATAC12760.30680083192075114Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12130.29165314194347264Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT12030.28924874670898404Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTAC11600.27890984720068285Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGCTCCCACTGCTTCCCATGTACTCTGCGT11430.27482237530205217No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11420.2745819357786033No Hit
CTTATACACATCTCCGAGCCCACGAGACATAATACCAGCGATCTCGTATG10960.2635217176999555No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC9830.23635205155023384Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT9440.22697491013572813Clontech Universal Primer Mix Long (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC9400.22601315204193265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9120.21928084538536444Clontech Universal Primer Mix Long (96% over 26bp)
CTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTA9060.21783820824467126Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTC8830.2123080992053474Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTG8240.19812216732186436Clontech Universal Primer Mix Long (95% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8170.19643909065772233Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTAC8070.19403469542323368Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8040.19331337685288708No Hit
ACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7940.19090898161839845Clontech Universal Primer Mix Long (95% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7610.1829744773445859Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT7610.1829744773445859Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7560.18177227972734158Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6840.16446063403902333Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.16349887594522788No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC6790.163258436421779Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACATAATACCAGCGATCTCGTATGCCG6660.16013272261694378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.15820920642935285No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6400.1538812950072733Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG6230.1497938231086426Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA6130.14738942787415396No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5970.1435423954989721Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCC5890.1416188793113812Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5860.1408975607410346Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT5760.13849316550654597Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACATAATACCAGCGATCGCGTATG5730.13777184693619937No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA5620.13512701217826187No Hit
CTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTT5610.134886572654813Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5360.12887558456859138Clontech Universal Primer Mix Long (95% over 21bp)
GTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5170.12430723362306295Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACACATCTCCGAGCCCACGAGACATAATACCAGCGATCTCGTATGCCGTC5100.1226241569589209No Hit
AAAAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCT5080.12214327791202319Clontech SMART CDS Primer II A (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4940.11877712458373908No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4800.11541097125545498Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCAC4770.11468965268510838Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4640.11156393888027315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCA4630.11132349935682427Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA4630.11132349935682427Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGT4580.11012130173957996Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4490.10795734602854017Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTG4440.10675514841129584Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4290.10314855555956287Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGCTCCCACTGCTTCCCATGTACTCTGC4240.10194635794231856No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4200.10098459984852311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT4160.10002284175472764Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)