FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003637889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGCTAATGAGACTGGATTTTTGTTTTTTATGTTGTGTGTCA22.1739130434782608No Hit
GTTGGTTTCTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAA22.1739130434782608No Hit
GTGCTGAGCCTTGGCCCACTTTTTAATTAGGCGTTTGTCTTTTTATTACT22.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGTGGCTGCAGCTGTGCTCCTGCTCCCTCC22.1739130434782608No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCCATTGCATTCCATTCCATTCGATTCCATTCCATTCCAATTCTTTCC22.1739130434782608No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTGAGAAAAAACACCACC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAAAAAAAAAAAC11.0869565217391304No Hit
GTGTATAGGAATGCTTGTGATTTTTGCACATTGATTTTGTATCCTGAGAT11.0869565217391304No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATTT11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATT11.0869565217391304No Hit
AATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAA11.0869565217391304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGTTAAAACCAA11.0869565217391304No Hit
GGGCCCACAGGAGCACACCACCACATCAGGCGCATTTTTTTTTGTAGTGA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTATAACCAG11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAACCAACCCACGAAAAC11.0869565217391304No Hit
GACTCGAATGGAATAGAAACAAATGGAATGGAATGGAATGCAAAGGAATA11.0869565217391304No Hit
AAGTGAGACGCAGAGAGCGCCTTCCAGTGATCTCATTGACTGATTTAGAG11.0869565217391304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGAAGCA11.0869565217391304No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACGCCGTGGTCTGCATCTCGTATGCCGTCTTCTG11.0869565217391304TruSeq Adapter, Index 18 (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GAGCCACCGCACCTGGTCATGACTCTAATCTTTAAGTCAATATTTATCAT11.0869565217391304No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
ACATCTCCGAGCCCACGAGACGCCGTGGTCTGCATCGCGTATGCCGTCTT11.0869565217391304No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCAAGGATTGCCACAACAGAAGCAAACTCTTGGGTTAATGGGAGGAT11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACACCCATGT11.0869565217391304No Hit
CTTTAAGCAGTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCA11.0869565217391304No Hit
GCAGAGTACATGGGACAGTTAAATCTCCAGCTGGCCAATAGCCTAGGCTC11.0869565217391304No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACGCCGTGGTCTGCATCTCGTATGCCGTCT11.0869565217391304No Hit
GGGCCCACAGGAGCACACCACCACATCAGGCTCATTTTTTTTTGTAGAGA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAACCAAACCCCC11.0869565217391304No Hit
CCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAA11.0869565217391304No Hit
CATCTCCGAGCCCACGAGACGCCGTGGTCTGCATCGCGTATGCCGTCTTC11.0869565217391304No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTAAAAAAACCCATGTA11.0869565217391304No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAACCAACCCAA11.0869565217391304No Hit
GACTCCTACGGGAGGCAGCAGAAAGGAGGTGATCCAGCCGCACCTTCCGA11.0869565217391304No Hit
CCCATGTACTCTGCTTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT11.0869565217391304No Hit
CTACACTCTTTCCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTAC11.0869565217391304Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACCATACTATGATTGCATTCAATTCATTCGATGACCATTCCATTCAATTC11.0869565217391304No Hit
ACATCAAGTAGTCCATAAGCATCGGTGAGGCGGCCCGCGGGTCGCCCCAC11.0869565217391304No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTAAGTTGACTTCATATCTTGGTTATTGTGAATAGTGCTGCAATAG11.0869565217391304No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAACAC11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTAATGTTTAT11.0869565217391304No Hit
TCCCACTTCGTTTCCCACTTAGCCAATTTTAGGGACCTTAGCTGGCGGTC11.0869565217391304No Hit
ATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGC11.0869565217391304No Hit
ACACTGCGGAGGCCGGCAGGGCAGCGGCTGCCGCCAAGCACACCACTCAT11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA11.0869565217391304No Hit
GGGGGTGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0869565217391304No Hit
TAGCAGTCCTGCGTGTAGAAGTGGCCAAACTCCTCTTCCGGCAGCCGCGC11.0869565217391304No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA11.0869565217391304No Hit
TCCCAAATAAATGCCATAAATGAATTTGAAAGATGAGTAACACACGAAAA11.0869565217391304No Hit
GGGGCCGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0869565217391304No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGCGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0869565217391304No Hit
GAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTCTCGGCAGGCTCTTCGGGAAGGGTGAGCCAGGGTGGGACTGGCCA11.0869565217391304No Hit
GGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGATGCC11.0869565217391304No Hit
AACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGC11.0869565217391304No Hit
GTTGTAGATATGCGGCGTTATTTCTGAGGGCTCTGTTCTGTTCCATTGAT11.0869565217391304No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCC11.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC11.0869565217391304No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA11.0869565217391304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.0869565217391304Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GATACACGCTGATGCACGTAGTGAGCATCCTGTGCTCGCATATACCGCTG11.0869565217391304No Hit
CACATCTCCGAGCCCACGAGACGCCGTGGTCTGCATCGCGTATGCCGTCT11.0869565217391304No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAAACAAAAA11.0869565217391304No Hit
CGGCAGGCCCCTAAACCCAAAAGTCGGGTTCTACAGGGTATATGATGTTG11.0869565217391304No Hit
GTCTGGAACTCCTGGCCTCAAACAATTCTCCTGCCTCACCCTATCAAATA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAAAGATAA11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)