FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003637919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA714.755525787006029Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT523.4829202947086406Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG302.0093770931011385Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATG261.7414601473543203No Hit
ATACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATGCCG251.6744809109176158No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG241.6075016744809107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT181.2056262558606832Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCGCGTATG171.1386470194239784No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG171.1386470194239784Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATGCCGTC151.0046885465505693No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC110.7367716008037508Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.6697923643670463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC100.6697923643670463Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCGCGTATGCCG100.6697923643670463No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90.6028131279303416Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90.6028131279303416Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80.5358338914936369Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC80.5358338914936369Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA80.5358338914936369Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC60.4018754186202277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.4018754186202277Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGGTTAGGATACCATCGCGTATGCCGTC50.33489618218352313No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA50.33489618218352313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT50.33489618218352313No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC50.33489618218352313Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGGTTAGGATACCATCGCGTATGCCGTCTTC50.33489618218352313No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA50.33489618218352313No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50.33489618218352313Clontech SMART CDS Primer II A (96% over 26bp)
CATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATGCCGTCTTC40.26791694574681846RNA PCR Primer, Index 14 (95% over 22bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAG40.26791694574681846No Hit
CACATCTCCGAGCCCACGAGACGGTTAGGATACCATCGCGTATGCCGTCT40.26791694574681846No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.26791694574681846No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.20093770931011384Clontech Universal Primer Mix Long (100% over 23bp)
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA30.20093770931011384No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30.20093770931011384Clontech Universal Primer Mix Long (96% over 25bp)
ACATGGGACAGTGTCCTCCCTGGACTGATTCTGTGGTATGGCTTTGGCTT30.20093770931011384No Hit
GGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTC30.20093770931011384No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
CAGCTCGAGCGCGCATACATCCTGCGGATGCTGTCGGCGGTGGCACCGAG30.20093770931011384No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.20093770931011384Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
GGTATAAGGTGATATCTCATTGTGGTTTTTATTTTCATTTCCCTGATGAC30.20093770931011384No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT30.20093770931011384No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG30.20093770931011384No Hit
CACATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATGCCGTCT30.20093770931011384No Hit
AGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATC30.20093770931011384No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC30.20093770931011384No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC30.20093770931011384No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
GAATGGAATGCAATGGAATGGATTCAACTTGAATGGAATGGAAAGAATGG20.13395847287340923No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
CAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTAC20.13395847287340923No Hit
GACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCC20.13395847287340923No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT20.13395847287340923No Hit
GTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGG20.13395847287340923Clontech Universal Primer Mix Long (96% over 27bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20.13395847287340923No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA20.13395847287340923No Hit
GCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTCTCAAGATAAGGA20.13395847287340923No Hit
GCTTAATACGGATGCCCAGAGGTTTAGCCGGATCACCGTGTGATGTAGCG20.13395847287340923No Hit
GCCGGGCGCCGAGGTGAGCTGGTCGGAATACACGGTCTGGATCGAGTCGA20.13395847287340923No Hit
GTTAATTCCAGGGACTGTTTTCTTTCCAATGGACTGTTTGTTGGTAGAAT20.13395847287340923No Hit
GCAACAGACTCTCCTCCCTTGCTGAGACCAGAAGGTGAGTGAGGGCTTTG20.13395847287340923No Hit
GATACATTCCTCGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGT20.13395847287340923No Hit
GGCGGGTGCCTGTGGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGG20.13395847287340923No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC20.13395847287340923No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG20.13395847287340923No Hit
CCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAG20.13395847287340923No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA20.13395847287340923No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC20.13395847287340923No Hit
ATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTC20.13395847287340923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20.13395847287340923No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT20.13395847287340923No Hit
ACATGGGAAGTTCAGATTCTTCCCATGTTCCATGGCTCAGCTCCAGACCC20.13395847287340923No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC20.13395847287340923No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT20.13395847287340923No Hit
CCTCAAGGCTGGCGTCACCGGAGCCCCTGCCAGGCAGCAGCGAGGATGGT20.13395847287340923No Hit
GTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAG20.13395847287340923No Hit
GTCTGAGTTGGTACTGAGCACCCAACGTGTGGAAGGTACCATCTGCGAAG20.13395847287340923No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC20.13395847287340923No Hit
CACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
CACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGC20.13395847287340923No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT20.13395847287340923No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
CAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCA20.13395847287340923No Hit
TCTCCATCACGCACGTGTCTGACTCGGACGCCGAGTCCAAGAGAGGACCT20.13395847287340923No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.13395847287340923Clontech SMART CDS Primer II A (96% over 26bp)
TCTCTATAGGAAGGTGCTGGGCAGGGATCCCAGAGAAAGAAAGGGTCCAA20.13395847287340923No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT20.13395847287340923No Hit
GTTTGAATCTGGGCCTAGAGATCAGAGACAGAAGTTAGGAATTCATATCA20.13395847287340923No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTT20.13395847287340923No Hit
CAGTAGTACACGTCATCTGGTTGAAGTGCAAAATATTTGTACAAGTATGC20.13395847287340923No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.13395847287340923No Hit
GGATCACGAAGATCTTGCCGTCGCCGATCCGGCCGGTCTGCGCCGCGGCG20.13395847287340923No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGT20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT20.13395847287340923No Hit
CCCTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAA20.13395847287340923No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG20.13395847287340923No Hit
GATTGGGAGGAGAGGGAGATGCTGGTGACTGGAGATGGTGAGAGCCTGGG20.13395847287340923No Hit
GAAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTC20.13395847287340923No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA20.13395847287340923No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA20.13395847287340923No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
TTTGTTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC20.13395847287340923No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG20.13395847287340923No Hit
AAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCC20.13395847287340923No Hit
GATTTGTGTTGGTGTTCTCATTTGTCTAATTACCAACAGGATAAACAACT20.13395847287340923No Hit
GTACTAAGCATCTCTAACTCGTACTAGCGATCTGATAGACTGCTACCAGC20.13395847287340923No Hit
TAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCG20.13395847287340923No Hit
GTTCAAGACCCTATGGAACGGGCAGAAGTTGGTCACCACAGTGACAGAAA20.13395847287340923No Hit
GTATTAGTCCATTCCCTGCATTGCTATGAAGAATACCTGGGACTGGGTAA20.13395847287340923No Hit
GGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC20.13395847287340923No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
CCATTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20.13395847287340923No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG20.13395847287340923No Hit
GTACATGGGAAGACTTCCCTTTGGGTAAGGCTAGGTCTTCAGTGCACAGC20.13395847287340923No Hit
CCATTGGATGATGATTCCTTTCGATGCCATTCAATGATTCCCTTTGATTT20.13395847287340923No Hit
CTTGATCACCTCTTGATCTCAAACCCCTTTCTCTTCCCCTTCCCAACATA20.13395847287340923No Hit
GCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCT20.13395847287340923No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC20.13395847287340923No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA20.13395847287340923No Hit
GAATAGGGGTTGTGCGCGGTAACGTGCCTCCGCGACTAGCTACCCAGGGC20.13395847287340923No Hit
TTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTA20.13395847287340923No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
TCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGG20.13395847287340923No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20.13395847287340923No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
ACATCTCCGAGCCCACGAGACGGTTAGGATACCATCTCGTATGCCGTCTT20.13395847287340923RNA PCR Primer, Index 24 (95% over 21bp)
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT20.13395847287340923No Hit
GTCTTGATCTGTCACTTAGGCTGGAGTGCAGTGGCATAATCACAGCTCAC20.13395847287340923No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)