FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003637920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003637920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15810.58271935699933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA835.5592766242464835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT422.813127930341594Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG332.2103148024112524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT191.2726054922973877Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC191.2726054922973877Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG181.2056262558606832Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161.0716677829872738Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG140.9377093101138647Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG130.8707300736771602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC130.8707300736771602Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.6697923643670463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70.46885465505693236Clontech Universal Primer Mix Long (96% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT70.46885465505693236No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.4018754186202277Clontech Universal Primer Mix Long (100% over 23bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC60.4018754186202277Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60.4018754186202277Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.4018754186202277Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG50.33489618218352313Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC50.33489618218352313Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA40.26791694574681846Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.26791694574681846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT40.26791694574681846No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.26791694574681846Clontech Universal Primer Mix Long (96% over 26bp)
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG30.20093770931011384No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA30.20093770931011384No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT30.20093770931011384No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.20093770931011384Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC30.20093770931011384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.20093770931011384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.20093770931011384Clontech SMART CDS Primer II A (96% over 26bp)
GTGTGATGCTCTTATAAGAAGCCACAAATACCCTGTATGGGATGTCACAA30.20093770931011384No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.20093770931011384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA30.20093770931011384No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG30.20093770931011384No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
GCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA30.20093770931011384No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
CCATCATGGATGTGGTGGCGCAGGCTGCAGGCGTTGCCGCCGAGGACATC30.20093770931011384No Hit
GGAGTATACATTGCTGTTTCCTGTTGGGCAATGCTCCTTGACCACATTTT30.20093770931011384No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.20093770931011384Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC30.20093770931011384No Hit
GATCTACACAATACTAAATATTTCTTCCATCTGACTTTGGATTCATACTC20.13395847287340923No Hit
TTCCCGTGTTGCGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC20.13395847287340923No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG20.13395847287340923No Hit
GAATGGAATCCAATGTAATCATCATTGAATTGAACCCAATGGAATCATTA20.13395847287340923No Hit
GTCTCGGGGCCATGCCTCGTCCTGGTTTAAGTGTCCTGTGAGCACACCGG20.13395847287340923No Hit
CCTTTAGGCTAGGCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTC20.13395847287340923No Hit
GGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGC20.13395847287340923No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATAC20.13395847287340923No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATCC20.13395847287340923No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG20.13395847287340923No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA20.13395847287340923No Hit
CATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAG20.13395847287340923No Hit
AAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGA20.13395847287340923No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC20.13395847287340923No Hit
ACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCT20.13395847287340923No Hit
CTTCTTGCGTTTAAGCGGGAAAGATCGCGAGGGCCCGCTATTTGCGATAC20.13395847287340923No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
AAGAAAATATGCATGTCTTGTTTCTTAGTAATCTATCTAGTTCATTGTTA20.13395847287340923No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT20.13395847287340923No Hit
GTATCAAGACGGTTCTTTTCTAGATTTCCTCTACCTTTTTGCTCTTAAAA20.13395847287340923No Hit
AGCCAAGGCCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGA20.13395847287340923No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAATGAATTCCCAGTTCCTGAGCAGTTCAAGACCCTATGGAACGGGC20.13395847287340923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA20.13395847287340923No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA20.13395847287340923No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
GCTCTGGCTCTCTGTGTGACCTTGGGCAGCTCCGTGCCTCTCTCTGTACT20.13395847287340923No Hit
GCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
CCACTCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
CCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGGGCTGCGTGCGTC20.13395847287340923No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT20.13395847287340923No Hit
CCATGGGATAAATCTGTATCAAGACGGTTCTTTTCTAGATTTCCTCTACC20.13395847287340923No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATGATGCTGGCCTCATAAAATGAGTTAGGGAGGATTCCCTCTTTTTCT20.13395847287340923No Hit
TCAATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTA20.13395847287340923No Hit
AAAAGGGCCTGGTTCAGCTTTTTCTCCAGGGCCATGGCAGCTTTCATGGC20.13395847287340923No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.13395847287340923No Hit
CCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTA20.13395847287340923No Hit
GCATGGGAGTGATCTATCCTGCCAGGAGGCCTCAGAGTGATCTGTCCTGC20.13395847287340923No Hit
GTTGATACCACTGCCTTCCCATGTACTCTGCGTTGATACCACTGCCCATG20.13395847287340923Clontech SMART CDS Primer II A (95% over 24bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.13395847287340923Clontech Universal Primer Mix Long (96% over 25bp)
TACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAA20.13395847287340923No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC20.13395847287340923No Hit
GTGTTAAGGGAGGAACATGTTGGGAAGTGATTTAATCATGGGGGTGGTTT20.13395847287340923No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTAATCCTTTTCAGCCTACAGTCCAGCCCTCACAAGCTTCCCAAAGC20.13395847287340923No Hit
GATACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTGGGAGGTGGAGCCTTTGGGAAGGTTATTAGATCATGACAGTGGGT20.13395847287340923No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC20.13395847287340923No Hit
GAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCG20.13395847287340923No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT20.13395847287340923No Hit
CATCGCACCCCGGTACGCTCGTCGCCCCAACGCGATTCCGCCGAAACGGT20.13395847287340923No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
GGCATCGCCCCTCGTTTATGAACTAGCCTATCGCAGACGGTATCAACAGG20.13395847287340923No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.13395847287340923Clontech SMART CDS Primer II A (96% over 26bp)
ACTAGTTGACTTGGAGTATGCCATGAAGACTCGTCCCTCGATATCAATAC20.13395847287340923No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT20.13395847287340923No Hit
GCAGTGGTGAGTGGGTGAAGCGCAGTGACACGGCAGATTTTAGAGGCTGG20.13395847287340923No Hit
AGCTGGCGCAATGACGACCTAATTGGCGCACAGTACTAGGCATCGTCATC20.13395847287340923No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT20.13395847287340923No Hit
CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA20.13395847287340923No Hit
GGTTGACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACCACAGG20.13395847287340923No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA20.13395847287340923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.13395847287340923No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA20.13395847287340923No Hit
GCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCTTT20.13395847287340923No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG20.13395847287340923No Hit
CGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACT20.13395847287340923No Hit
GACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCA20.13395847287340923No Hit
GACCTGTGGCCTCCCCACTCTGGTCCAGACCTATGTGCTCCAGGGGGACC20.13395847287340923No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20.13395847287340923No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.13395847287340923Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20.13395847287340923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGA20.13395847287340923No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT20.13395847287340923No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA20.13395847287340923No Hit
AAAGTGATCCTTTTTTTATGCCCGAGATATGCAAAAGTTATAAGGGAGAG20.13395847287340923No Hit
CTACTTGGGAGGATCACTTGAGCCAGGGAGGTTGAGGCTGCAGTGAGCTG20.13395847287340923No Hit
GTACATGGGAGTAGCCGCCTCTGCCGCCGCGGAGCTTCCCGAACCTCTTC20.13395847287340923No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTTCCCATGT20.13395847287340923Clontech Universal Primer Mix Long (96% over 27bp)
ATATAACATATGAGGTAGTAAAATGTATGAAAACAAGATAAAGGGTAGGA20.13395847287340923No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)