FastQCFastQC Report
Tue 10 May 2022
EGAF00003638039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171656
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103416.024257817961504Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37982.2125646642121453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37852.204991378105047Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36322.1158596262292027Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23371.3614438178682946Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22451.3078482546488326Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18011.04919140606795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13440.7829612713799693Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10250.5971244815211819Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9700.56508365568346Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9520.5545975672274782Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9420.5487719625297106Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8370.4876031132031505Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7890.45964021065386584Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7870.45847508971431233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7400.4310947476348045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7210.42002609870904595Clontech Universal Primer Mix Long (96% over 26bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6230.36293517267092323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6150.35827468891270914No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5470.31866057696788924Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5330.31050473039101456Clontech Universal Primer Mix Long (96% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC4800.27962902549284613Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4460.25982196952043624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4030.23477186932003544Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4010.2336067483804819No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3950.2301113855618213No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3590.20913920864985783No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3530.2056438458311973Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3470.20214848301253668Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3040.1770983828121359Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.1689425362352612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.1677774152957077No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2570.14971804073262804Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2560.14913548026285128Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC2560.14913548026285128Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA2420.1409796336859766No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2400.13981451274642306Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2280.13282378710910192Clontech Universal Primer Mix Long (96% over 26bp)
GGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2270.13224122663932517Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2260.1316586661695484Clontech Universal Primer Mix Long (96% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2250.13107610569977163Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2250.13107610569977163Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2240.13049354522999487Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2220.12932842429044134No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG2220.12932842429044134Clontech Universal Primer Mix Long (96% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2220.12932842429044134No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA2180.1269981824113343Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.12292025912289696Clontech Universal Primer Mix Long (95% over 22bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2110.12292025912289696No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1930.11243417066691523No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1880.1095213683180314Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1870.10893880784825466Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1840.10719112643892437Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1840.10719112643892437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1820.10602600549937083Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1780.1036957636202638No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1780.1036957636202638No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1780.1036957636202638Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1780.1036957636202638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1750.10194808221093349Clontech SMART CDS Primer II A (100% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1730.10078296127137996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)