FastQCFastQC Report
Tue 10 May 2022
EGAF00003638065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117234
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.5936844260197554No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6180.5271508265520242No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCAGGCTCAGGATCTCGTATG4390.37446474572223076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.323285053823976No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3170.27039937219577936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.23713257246191377No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2580.2200726751624955No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2450.20898374191787367No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2190.18680587542862992No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCAGGCTCAGGATCGCGTATG2170.1850998856986881No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2140.18254090110377535No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2140.18254090110377535No Hit
ATACACATCTCCGAGCCCACGAGACTCCAGGCTCAGGATCTCGTATGCCG2060.17571694218400805No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2050.17486394731903715No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT2000.17059897299418258No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1910.16292201920944438No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1890.16121602947950253No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1870.1595100397495607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1800.15353907569476433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1780.15183308596482248No Hit
ACACATCTCCGAGCCCACGAGACTCCAGGCTCAGGATCTCGTATGCCGTC1600.13647917839534607No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1530.13050821434054966No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1450.12368425542078236Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1350.11515430677107323No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1340.11430131190610232No Hit
ATACACATCTCCGAGCCCACGAGACTCCAGGCTCAGGATCGCGTATGCCG1220.10406537352645137No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1190.10150638893153864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)