FastQCFastQC Report
Tue 10 May 2022
EGAF00003638304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125270
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74595.954338628562306No Hit
GTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGG10390.8294084776881936No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG8120.6481998882413986No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7810.6234533407839068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.6082861020196376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.464596471621298No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5800.4629999201724275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG5320.42468268539953696No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA4980.3975413107687395No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG4650.3711982118623773No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG4420.3528378702003672No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCC4420.3528378702003672No Hit
GTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGA4410.35203959447593197No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA4130.32968787419174583No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG3950.3153189111519119No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT3940.31452063542747666No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC3940.31452063542747666No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC3820.304941326734254No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3820.304941326734254No Hit
GGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTC3780.3017482238365131No Hit
GAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCAT3660.29216891514329046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.2873792607966792No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3590.28658098507224394Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3580.28578270934780875Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3560.2841861578989383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA3430.2738085734812804No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA3400.27141374630797477No Hit
TATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAG3360.2682206434102339No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT3230.25784305899257604No Hit
ACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTG3200.2554482318192704No Hit
CACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGG3180.25385168037039996No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG3160.2522551289215295No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT3150.25145685319709427No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT3030.24187754450387167No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG3000.23948271733056598No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG2950.2354913387083899No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC2950.2354913387083899No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC2930.23389478725951945No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC2860.22830685718847288No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA2790.22271892711742636No Hit
ACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCC2720.2171309970463798No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG2680.21393789414863892No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2550.20356030973098108Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC2540.20276203400654588No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC2500.199568931108805No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG2400.19158617386445279No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG2390.19078789814001756No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG2340.18679651951784146No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC2330.18599824379340624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2320.18519996806897104No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT2310.18440169234453582Clontech Universal Primer Mix Long (96% over 26bp)
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC2280.18200686517123016No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2260.18041031372235972No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA2170.17322583220244273No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC2090.16683962640696096No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG2090.16683962640696096No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA2080.16604135068252573No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2080.16604135068252573Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC2040.16284824778478485No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC1970.15726031771373833No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.15486549054043267No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC1930.15406721481599744No Hit
GGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCA1930.15406721481599744No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1900.15167238764269178Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATG1900.15167238764269178No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1880.15007583619382134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1850.14768100902051567No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT1790.14289135467390437No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC1760.1404965275005987No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC1750.1396982517761635No Hit
GGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAG1730.13810170032729305No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT1700.13570687315398738No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA1670.13331204598068172No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG1670.13331204598068172No Hit
GGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCG1660.1325137702562465No Hit
GGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGC1650.1317154945318113No Hit
ACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT1650.1317154945318113No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1620.12932066735850564No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG1610.12852239163407042No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG1610.12852239163407042No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG1610.12852239163407042No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC1570.12532928873632954No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1570.12532928873632954No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC1550.1237327372874591No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.1237327372874591No Hit
GGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCC1520.12133791011415343No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT1500.11974135866528299No Hit
ATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGG1450.11574998004310688No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1430.11415342859423644No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA1410.112556877145366No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC1400.11175860142093079No Hit
GGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGG1390.11096032569649557No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT1370.10936377424762513Clontech Universal Primer Mix Long (96% over 26bp)
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT1370.10936377424762513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1340.10696894707431946No Hit
GGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCAC1340.10696894707431946No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.10297756845214338No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1290.10297756845214338No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1280.10217929272770816Clontech SMART CDS Primer II A (100% over 26bp)
GCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCG1280.10217929272770816No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA1270.10138101700327294No Hit
ACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGA1260.10058274127883772No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1260.10058274127883772Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)