FastQCFastQC Report
Tue 10 May 2022
EGAF00003638319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55515
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATG9961.794109700081059No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCGCGTATG6101.0988021255516527No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCG5811.0465639917139513No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5801.0447626767540303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.9240745744393408No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCGCGTATGCCG3960.7133207241286138No Hit
ACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCGTC3410.6142484013329731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.6124470863730523No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3290.5926326218139242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCGCGTATGCCGTC3010.5421958029361433No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2590.46654057461947224No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2430.43771953526074037Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2280.4106998108619292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.3602629919841484No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1910.34405115734486175Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1870.3368458975051788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1760.31703143294605063No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1740.3134288030262091No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1550.279203818787715No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1540.2774025038277943No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1520.2737998739079528Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1450.2611906691885076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1430.25758803926866614No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.24497883454922093No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1310.23597225974961722Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1260.2269656849500135Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1220.21976042511033053No Hit
CACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCGTCT1220.21976042511033053Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1180.2125551652706476No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1160.2089525353508061No Hit
CATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCGTCTTC1140.2053499054309646Illumina PCR Primer Index 8 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1140.2053499054309646Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1090.19634333063136092No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1030.18553544087183643No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1010.18193281095199496No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1010.18193281095199496No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1000.1801314959920742No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1000.1801314959920742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA990.17833018103215345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG990.17833018103215345No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.16391966135278754No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC910.16391966135278754No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG900.1621183463928668No Hit
CACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCGCGTATGCCGTCT870.15671440151310456No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC850.15311177159326309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT830.1495091416734216No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA830.1495091416734216No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT820.14770782671350086Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA800.14410519679365938No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC790.14230388183373863No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA780.14050256687381787No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA760.1368999369539764No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC750.13509862199405567No Hit
CATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCGCGTATGCCGTCTTC740.13329730703413492No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.13329730703413492No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA690.1242907322345312No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA690.1242907322345312No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA680.12248941727461046No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT670.12068810231468972Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT670.12068810231468972No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA670.12068810231468972No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC660.11888678735476899No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT650.11708547239484823Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA640.1152841574349275No Hit
ACATCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCGTCTT620.11168152751508602Illumina PCR Primer Index 8 (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC620.11168152751508602Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG610.10988021255516527No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC610.10988021255516527No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG610.10988021255516527No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA610.10988021255516527No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT610.10988021255516527No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG580.10447626767540305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT580.10447626767540305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA570.1026749527154823No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA560.10087363775556156No Hit
TCTCCGAGCCCACGAGACCGGTTTCCTTGCATCTCGTATGCCGTCTTCTG560.10087363775556156Illumina PCR Primer Index 8 (96% over 25bp)
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA560.10087363775556156No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT560.10087363775556156Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)