FastQCFastQC Report
Tue 10 May 2022
EGAF00003638361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37404
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATG9622.5719174419848145No Hit
ATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCG9172.451609453534381No Hit
ACACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCGTC6071.6228210886536198No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATG5111.3661640466260294No Hit
ATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCCG4571.2217944604855095No Hit
ACACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCCGTC3660.9785049727301893No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2840.7592770826649556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.5560902577264464No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1980.5293551491819056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1980.5293551491819056Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCGTCT1890.505293551491819Illumina PCR Primer Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.4518233344027377No Hit
CATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCGTCTTC1440.384985563041386Illumina PCR Primer Index 1 (95% over 23bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1300.34755641107902896No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1150.30745374826221794No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1130.30210672655330983No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1130.30210672655330983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1110.2967597048444017Clontech SMART CDS Primer II A (100% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC950.2539835311731366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.2406159769008662No Hit
CACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCCGTCT900.2406159769008662No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG890.23794246604641214No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC870.232595444337504No Hit
TTATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGC820.2192278900652337No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA800.21388086835632553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG780.2085338466474174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG780.2085338466474174No Hit
TATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCC760.20318682493850926No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA760.20318682493850926No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT730.19516629237514704Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA720.192492781520693No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1898192706662389No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT700.18714575981178483No Hit
CATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCCGTCTTC670.1791252272484226RNA PCR Primer, Index 22 (95% over 22bp)
TCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCGTCTTCTG660.17645171639396856Illumina PCR Primer Index 1 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG640.17110469468506043No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT640.17110469468506043Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT630.16843118383060635No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA630.16843118383060635No Hit
ACATCTCCGAGCCCACGAGACCACCTATTTACGATCTCGTATGCCGTCTT600.16041065126724416Illumina PCR Primer Index 1 (95% over 22bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC590.15773714041279008No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG550.14704309699497378Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA540.14436958614051973No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT540.14436958614051973Clontech SMART CDS Primer II A (100% over 26bp)
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT540.14436958614051973No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG530.14169607528606565Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC530.14169607528606565Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA520.1390225644316116No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT510.13634905357715754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG510.13634905357715754No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC500.13367554272270346No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT500.13367554272270346Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA470.12565501015934125No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG460.12298149930488718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT460.12298149930488718No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG450.1203079884504331No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA440.11763447759597903No Hit
ACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCCGTCTT440.11763447759597903No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA430.11496096674152498No Hit
TATACACATCTCCGAGCCCACGAGACCACCTATTTACGATCGCGTATGCC430.11496096674152498No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC430.11496096674152498No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA430.11496096674152498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT420.11228745588707091No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA420.11228745588707091No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC410.10961394503261684Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA410.10961394503261684No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA410.10961394503261684No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA410.10961394503261684No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC410.10961394503261684No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA400.10694043417816276No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA400.10694043417816276No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10694043417816276No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT400.10694043417816276No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG390.1042669233237087No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)