FastQCFastQC Report
Tue 10 May 2022
EGAF00003638383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92274
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATG21042.2801655937750613No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATG15131.63968181719661No Hit
ATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCG14071.5248065543923532No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12501.3546611179747274No Hit
ATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGCCG11241.218111277282875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10051.089147538851681No Hit
ACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCGTC9361.0143702451394758RNA PCR Primer, Index 7 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.9298393913778529No Hit
ACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGCCGTC7830.8485597242993692No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA5500.59605089190888No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5100.5527017361336888No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT5060.5483668205561697Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT4430.48009190021024345Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.4692546112664456No Hit
CATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCGTCTTC3670.39772850423737993RNA PCR Primer, Index 7 (96% over 25bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA3510.3803888419273035No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3330.3608817218284674No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3180.3446257884127707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT2870.31103019268699744Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.30994646379261764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2810.30452781932071876No Hit
CACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCGTCT2630.28502069922188267TruSeq Adapter, Index 7 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA2590.28068578364436353No Hit
CACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGCCGTCT2420.26226239243990723No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2110.22866679671413398No Hit
CATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGCCGTCTTC2060.22324815224223507No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2050.2221644233478553No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2020.21891323666471596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1990.21566204998157662No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1950.2113271344040575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1920.20807594772091811Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1820.19723865877712032No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1820.19723865877712032No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.19507120098836075No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.19290374319960119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1780.19290374319960119No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.17881526757266403No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1580.17122916531200555No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA1560.16906170752324598No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1490.16147560526258753No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1410.15280577410754925Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.15172204521316948No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1330.144135942952511No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT1320.14305221405813123Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCGTCTTCTG1320.14305221405813123RNA PCR Primer, Index 16 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA1320.14305221405813123No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1310.14196848516375143No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1290.13980102737499187No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1290.13980102737499187Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1280.1387172984806121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG1280.1387172984806121No Hit
TTATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGC1260.13654984069185253No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1240.13438238290309296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1240.13438238290309296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1220.1322149251143334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1210.13113119621995362No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1200.13004746732557382Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1200.13004746732557382Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1190.12896373843119405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1170.12679628064243448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1170.12679628064243448Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1110.1202939072761558No Hit
ACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCCGTCTT1110.1202939072761558RNA PCR Primer, Index 16 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA1070.11595899169863667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.1148752628042569Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGC1050.11379153390987709No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1030.11162407612111754No Hit
TATACACATCTCCGAGCCCACGAGACCTATGCCAGACCATCTCGTATGCC1030.11162407612111754No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1010.10945661833235798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT990.10728916054359842No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA980.10620543164921864No Hit
ACATCTCCGAGCCCACGAGACCTATGCCAGACCATCGCGTATGCCGTCTT960.10403797386045906No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC960.10403797386045906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA950.10295424496607929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT950.10295424496607929No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC950.10295424496607929No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC940.1018705160716995No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA930.10078678717731972No Hit
CATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10078678717731972No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)