FastQCFastQC Report
Tue 10 May 2022
EGAF00003638396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260177
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86743.3338842403440734No Hit
GTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGG25700.9877890820479904No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG22150.8513435084577038No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC17350.6668537188144994No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT14970.5753775314497439No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT14230.5469353555464165No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA14040.539632634706373No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG13910.5346360362368695No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA13010.5000442006787686No Hit
GTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGA11780.4527686920826976No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC11350.4362414817604938No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC11250.4323979444762604No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT10790.41471767296878664No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC10770.41394896551193994No Hit
GGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTC10440.40126529247396964No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG10040.38589114333703595No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA9770.3755135926696057No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG9610.3693639330148322No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA9570.36782651810113887No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC9520.3659047494590221No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT9420.3620612121747887No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG9380.36052379726109535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.35860202861897866No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA9300.3574489674337086No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9090.3493775391368184No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT8930.3432278794820449No Hit
ACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTG8840.3397686959262348No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG8810.3386156347409648No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT8710.33477209745673137No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCC8710.33477209745673137No Hit
GTATCAACGCAGAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCA8680.3336190362714614No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC8670.333234682543038No Hit
GAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCAT8630.3316972676293446No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA8630.3316972676293446No Hit
TATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAG8590.3301598527156513No Hit
CACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGG7990.3070986290102507No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG7710.29633672461439714No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA7570.2909557724164703No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT7290.28019386802061674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.27750339192165335No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC7050.2709693785384565No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC7040.27058502481003316No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT6980.2682789024394931No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG6950.2671258412542231No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA6910.2655884263405297No Hit
ACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCC6830.262513596513143No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA6670.2563639368583695No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG6580.2529047533025594No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC6560.2521360458457127No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG6520.25059863093201934No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA6330.24329591009197585No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG6240.23983672653616578No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG6140.23599318925193233No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC5930.22792176095504216No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG5840.22446257739923203No Hit
TATCAACGCAGAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCAC5820.22369386994238538No Hit
GTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATG5720.21985033265815193No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC5710.21946597892972863No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC5700.21908162520130528No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC5670.21792856401603522No Hit
CTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAAC5550.21331631927495512No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC5370.20639795216333495No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG5350.2056292447064883No Hit
GACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCG5350.2056292447064883No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC5150.19794217013802143No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC5120.19678910895275142No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC4900.18833332692743787No Hit
GGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAG4870.18718026574216784No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG4840.1860272045568978No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG4770.18333672845793442No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG4740.18218366727266438No Hit
CCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGG4730.18179931354424103No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA4590.17641836134631425No Hit
AAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATA4580.1760340076178909No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG4550.17488094643262087No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC4370.16796257932100073No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA4310.16565645695046066No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC4270.16411904203676728No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG4230.1625816271230739No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC4170.16027550475253385No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT4120.15835373611041714No Hit
ATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGG4050.15566326001145372No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG3970.152588430184067No Hit
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC3950.15181972272722033No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG3860.14836053917141023No Hit
GCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGG3810.14643877052929352No Hit
ACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT3770.14490135561560016No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA3760.1445170018871768No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3750.14413264815875346Clontech SMART CDS Primer II A (100% over 26bp)
CTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGG3750.14413264815875346No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC3720.14297958697348342No Hit
GTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCG3720.14297958697348342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.1425952332450601No Hit
ACGCAGAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCA3700.14221087951663675No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG3660.14067346460294336No Hit
GCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCG3650.14028911087452003No Hit
CTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTG3630.13952040341767336No Hit
ACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGA3630.13952040341767336No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG3620.13913604968925No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG3610.13875169596082668No Hit
GGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACA3590.13798298850398No Hit
ATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATA3550.1364455735902866No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC3540.1360612198618633No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC3540.1360612198618633No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3530.13567686613343993No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA3520.13529251240501658No Hit
ACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGT3480.13375509749132322No Hit
GCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGG3450.1326020363060532No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC3450.1326020363060532No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG3420.13144897512078316No Hit
GACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGT3390.13029591393551315No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT3350.12875849902181977No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC3330.1279897915649731No Hit
AAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCAT3310.12722108410812638No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA3270.12568366919443302No Hit
GTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAA3260.12529931546600967No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT3250.12491496173758633No Hit
GAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGT3250.12491496173758633No Hit
GGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGC3200.12299319309546962No Hit
GGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCC3160.12145577818177625No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG3130.12030271699650621No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT3110.11953400953965954No Hit
GAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTC3110.11953400953965954No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG3100.11914965581123618No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC3100.11914965581123618No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC3070.11799659462596616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3060.11761224089754284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA3040.11684353344069613No Hit
GTACATGGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCA2990.11492176479857942No Hit
ACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC2960.1137687036133094No Hit
GGTATCAACGCAGAGTACATGGGACATTTGCTTCTGACACAACTGTGTTC2940.11299999615646272No Hit
ATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAA2940.11299999615646272No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC2920.11223128869961602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2920.11223128869961602Clontech SMART CDS Primer II A (100% over 26bp)
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC2910.11184693497119269No Hit
GCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGA2910.11184693497119269No Hit
GTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCT2900.11146258124276934No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC2880.11069387378592266No Hit
GGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGA2860.10992516632907597No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG2830.10877210514380593No Hit
GTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAA2810.10800339768695925No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2810.10800339768695925No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG2800.10761904395853593No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT2800.10761904395853593No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC2800.10761904395853593No Hit
GGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCAC2770.1064659827732659No Hit
GCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGG2710.10415986040272585No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC2660.10223809176060912No Hit
CATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGC2650.1018537380321858No Hit
CTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCC2620.10070067684691576No Hit
AGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCC2620.10070067684691576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)