FastQCFastQC Report
Tue 10 May 2022
EGAF00003638435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398471
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52531.3182891603153053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24430.613093550095239Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20190.506686810332496Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19130.4800851253918102Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14470.3631380953695501Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12130.3044136210665268Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10980.27555330249880167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10330.25924094852573965Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.23615269367155953No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6970.17491862644960363Clontech SMART CDS Primer II A (96% over 26bp)
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT6930.17391478928203058No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA6870.172409033530671No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6330.15885723176843483No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC6270.15735147601707528No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5890.14781502292513132Clontech Universal Primer Mix Long (96% over 26bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5840.146560226465665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.13225554682774907No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5150.12924403532502993Clontech Universal Primer Mix Long (100% over 23bp)
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT5110.12824019815745688No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5080.1274873202817771Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC4800.12046046010876575No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4670.11719798931415334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4640.11644511143847355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4630.1161941521465803Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4620.11594319285468706Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4560.11443743710332747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10941825126546223No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAACGCTAACGATCTCGTATG4320.10841441409788917No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4250.10665769905463636Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT4210.1056538618870633No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA4140.10389714684381046No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT3990.10013275746541153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)