FastQCFastQC Report
Tue 10 May 2022
EGAF00003638479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286776
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA264249.214160180768264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT138884.842804139816442Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98753.4434541244734564Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84862.959103969648785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT56201.9597176890674255Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG56181.9590202806371522Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC46501.6214746003849694Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43141.504309984099088Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39581.380171283510475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36171.2612631461489106Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG24240.8452590174910034Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG23070.8044606243200269Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21910.7640109353641866Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20780.7246073590537562Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20670.7207716126872541Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC18110.6315033336122967Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17940.6255753619549753Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC17800.6206935029430636Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17520.6109297849192401Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA17270.6022121795408263Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG17120.596981616313778Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16220.5655982369514883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC13770.4801657042430329Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12840.44773621223533355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC12510.43622897313582726Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12510.43622897313582726Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11900.4149580160124976Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11820.4121683822914051Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10510.3664881301085168Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10450.36439590481769746Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9750.33998660975813877No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA9480.33057159594945185Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8210.28628616062710965Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7680.26780483722487236Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT6610.23049348620526128Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.22456551454793985No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG6380.22247328925712054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6300.21968365553602814Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA6150.21445309230897983Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT5850.20399196585488324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5780.20155103634892738No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5700.19876140262783498Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA5620.19597176890674256Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5290.1844645298072363Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5090.17749044550450524Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG4820.16807543169581834Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4670.16284486846877005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4640.1617987558233604Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA4490.1565681925963121Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4450.1551733757357659Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4410.15377855887521968Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4090.14262002399085Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACATCTTATTCTATCTCGTATG4010.13983039026975758No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3900.1359946439032555Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3880.1352972354729824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.1307640806762072No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA3670.12797444695511478Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT3530.12309258794320306Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC3500.1220464752977934Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3470.12100036265238374No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG3260.11367757413451612Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.11158534884369682No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3120.10879571512260439Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3060.10670348983178508No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2950.102867743465283Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2930.10217033503500991Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA2880.10042681395932714No Hit

[WARN]Adapter Content

Adapter graph