FastQCFastQC Report
Wed 11 May 2022
EGAF00003638903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824033
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA221160.45845457524855243Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105950.21962950916795138Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGAGTCGCCCTGGCATGAGTCCCTCCGGGTGTTCCCGGCACACAGC90490.18758163553192939No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG81290.16851045587789304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG79660.16513154035223226Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG70200.14552139257753832No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG66960.1388050206124212No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG54400.1127687144760411Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA51490.10673641743329701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)