FastQCFastQC Report
Wed 11 May 2022
EGAF00003638904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003638904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824033
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA196160.4066307174930188Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT113470.2352181255808159Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111750.23165264416723516No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73520.1524036008874732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG73200.15174025550820236Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGAGTCGCCCTGGCATGAGTCCCTCCGGGTGTTCCCGGCACACAGC68760.1425363383708196No Hit
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG60900.12624291749247984No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50230.1041244950024181Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)