FastQCFastQC Report
Tue 10 May 2022
EGAF00003639077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696764
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA413855.939600783048492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG182262.615806786802992Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATG167702.4068407667445504No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG167002.39679432347251Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT157842.2653294372269523Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA144332.071433082076571Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG116881.677468985194413Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGCCG109701.5744211813469122No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100041.435780264192754Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT96741.3884184601959917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT87691.2585323007503257Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATG82181.1794524401375501No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA71051.0197139921121068Clontech Universal Primer Mix Long (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGCCGTC64020.9188189975371862No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC61260.8792073069217125Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA57870.8305538173614022Clontech Universal Primer Mix Long (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATGCCG56090.8050071473267849No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT48770.6999500548248762Clontech Universal Primer Mix Long (100% over 23bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43750.6279027045025288Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA42730.6132636014489842Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT41840.6004902664316756Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40320.5786751324695306Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA39190.5624573026160938Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT38900.5582952046891057Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC38900.5582952046891057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATGCCGTC38660.5548507098529775No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA38600.5539895861439454Clontech Universal Primer Mix Long (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC38570.5535590242894294Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA38470.5521238181077094Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36860.5290169985820163Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG33430.47978942654901807Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA33380.479071823458158Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32980.4733309987312777Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG32760.4701735451314936Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28830.413769942189895Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT27500.3946816999730181Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA25760.369709112411089Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24290.348611581539804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT23600.33870865888593554Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC23200.3329678341590553Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC23160.33239375168636726Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG22810.327370530050347Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22620.3246436383050789Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG22340.3206250609962627Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC22070.31675000430561856Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT21340.3062729991790621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC21190.30412018990648193Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGCCGTCTTC21090.30268498372476194RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.30225442187024587No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20970.30096273630669784Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20380.29249501983454945Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20210.2900551693256253Clontech SMART CDS Primer II A (100% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20180.2896246074711093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.2777123961628328Clontech SMART CDS Primer II A (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT18660.2678094735089643Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.26048992198219195No Hit
CACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGCCGTCT17680.25374445292810766RNA PCR Primer, Index 23 (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA17600.2525962879827316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA17370.24929531376477543Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.2281977828934905Clontech SMART CDS Primer II A (100% over 21bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15750.2260449736209104Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.2149938860216659Clontech SMART CDS Primer II A (100% over 24bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT13310.19102594278694077Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13140.18858609227801665Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.18628976238726455No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12880.1848545562055445Clontech SMART CDS Primer II A (100% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT12550.18011837580586826Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATGCCGTCT12350.17724796344242813No Hit
TTATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGC11750.16863672635210775No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT11520.1653357521341516Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT11430.16404406657060355Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10980.15758563875286322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATGCCGTCTTC10300.14782623671716677No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9870.1416548501357705Clontech Universal Primer Mix Long (95% over 22bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9860.14151132951759848Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9820.14093724704491048No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC9710.13935852024501838Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.13620106664523426Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9230.1324695305727621Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9140.13117784500921403No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC8720.12514997904598976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT8410.12070083988265753Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8100.11625170071932535Clontech Universal Primer Mix Long (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCTCGTATGCC8040.1153905770102933No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA8000.11481649453760527Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT7840.11252016464685316No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT7610.10921919042889701Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.10907566981072501No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.108501587338037No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA7220.10362188632018876Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT7160.10276076261115671Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7130.10233020075664069No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7050.10118203581126464Clontech Universal Primer Mix Long (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGAAGGGTAGAGGATCGCGTATGC6980.10017739148406059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)