FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003639269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATG635.0602409638554215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA584.658634538152611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCG534.257028112449799No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT332.6506024096385543Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTC332.6506024096385543No Hit
ATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCG292.3293172690763053No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG272.1686746987951806Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATG241.9277108433734942No Hit
ACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCGTC221.7670682730923692No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161.285140562248996Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCT151.2048192771084338No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT141.1244979919678715Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC141.1244979919678715Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131.0441767068273093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCTTCTG131.0441767068273093RNA PCR Primer, Index 33 (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC131.0441767068273093Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT131.0441767068273093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110.8835341365461846Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCTTC110.8835341365461846Illumina Paired End PCR Primer 2 (95% over 22bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC90.7228915662650602Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCGTCT80.642570281124498No Hit
ACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCTT70.5622489959839357RNA PCR Primer, Index 33 (95% over 22bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70.5622489959839357Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCGTCTTCTG70.5622489959839357Illumina PCR Primer Index 5 (95% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.48192771084337355Clontech Universal Primer Mix Long (100% over 23bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60.48192771084337355Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.48192771084337355Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG50.4016064257028112Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT50.4016064257028112No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC50.4016064257028112Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.4016064257028112Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.4016064257028112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCGTCTT40.321285140562249No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCC40.321285140562249No Hit
CATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGCCGTCTTC40.321285140562249No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
GCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAAAT40.321285140562249No Hit
ACATAGGCATGCACTACATCACGACTCGACAGCCGGGGACGACCTTGCTC30.24096385542168677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCC30.24096385542168677No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCGAGTCCAAGTTCTTCTAGTCTCGCTTTCACTTCTTCAAGTGATTTG30.24096385542168677No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA30.24096385542168677No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC30.24096385542168677No Hit
ACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
AATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTC30.24096385542168677No Hit
CTTATACACATCTCCGAGCCCCGAGACCAATAGTAGCCGATCTCGTATGC30.24096385542168677No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GTAGAAAACTGGCTCGTCGTCCCAGACGCCCGATAGGAATGTGTACGGGT30.24096385542168677No Hit
TTATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGTATGC30.24096385542168677No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CGGACGGGGTGGTTGAAGCCGCGATGGCCGAAACGCAGCTTATAGGTATC30.24096385542168677No Hit
GTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGA30.24096385542168677No Hit
CCACCGTCGACGTGGCAGGCACACAAATCCTGCACGCCCACGGACACCAA30.24096385542168677No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCC30.24096385542168677No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.24096385542168677Clontech SMART CDS Primer II A (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTACAAGACCCGGGAACGTATTCACCGTGACATTCTGATCCACGAT20.1606425702811245No Hit
TTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGG20.1606425702811245No Hit
CTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCTTCTGC20.1606425702811245Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.1606425702811245Clontech Universal Primer Mix Long (96% over 25bp)
CGCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTATGCCGTCTTCTGC20.1606425702811245RNA PCR Primer, Index 33 (96% over 26bp)
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT20.1606425702811245No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA20.1606425702811245No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA20.1606425702811245No Hit
CAACGCTTCAAGATCGCCGGGAACGGGTCATCGCAGAACGGTTCCGGTCA20.1606425702811245No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.1606425702811245Clontech Universal Primer Mix Long (96% over 28bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.1606425702811245Clontech Universal Primer Mix Long (96% over 25bp)
GTCCGAAGCGTCGGATGCGTCAGTCCCAGGTTGAGAATACGGTCAGTCCT20.1606425702811245No Hit
GCTTGAGCTGGTCGGCGTTGGAGCCGGCGGTGCCGCCGATCACCAGGTTC20.1606425702811245No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT20.1606425702811245No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA20.1606425702811245No Hit
GAGTACATGGGCGGCCGTGCAGCGCGACGAAGCAGCGCGCGAAGCCGTCG20.1606425702811245No Hit
CGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACAT20.1606425702811245No Hit
CCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGT20.1606425702811245No Hit
ATCGATATCGGCGCGGATCTGCGCCGCCGTGCGGGTCCCTGGCACCGACG20.1606425702811245No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG20.1606425702811245No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.1606425702811245Clontech Universal Primer Mix Long (96% over 25bp)
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG20.1606425702811245No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.1606425702811245Clontech Universal Primer Mix Long (96% over 25bp)
ATTCTCGGCGGGTCCACCTGCGGGCTGGAGGAGGAGGCGGTCGCCTTCTA20.1606425702811245No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCGCGGATG20.1606425702811245No Hit
GTACATGGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGC20.1606425702811245No Hit
CCGTCATCGAGCGCAGGCCCACCGCGCTGTTCGCGCTGGTCATCACCAGC20.1606425702811245No Hit
GAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCG20.1606425702811245No Hit
TGTTCACCGTACGGGTCGAAATGCCTACCTTCGAGCAGCCTGCCAGAACC20.1606425702811245No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC20.1606425702811245No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGGGATCAGCTTGGAAATCCTGCCTGACCGTGCACGGGGGCTGCTG20.1606425702811245No Hit
ATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGGATGCCG20.1606425702811245No Hit
GGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA20.1606425702811245No Hit
AATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTA20.1606425702811245No Hit
GAGTACATGGGGGGAATTCGAGCTCTTTCGACGTTTTGAAGGAGGGTTTT20.1606425702811245No Hit
TATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTA20.1606425702811245No Hit
TGATGGGGCCATGTATTTCACTGACAGAGGTATCGAGGAACTAGAAGACC20.1606425702811245No Hit
CCCCCGTGCGGGAGACAACCCGTACACCGATAACCGGCGATGCACAACCG20.1606425702811245No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAATAGT20.1606425702811245No Hit
CTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAA20.1606425702811245No Hit
GAACGAAAACCAACCACGAATCACATGATACGCCCGAGAATCTTCGCCCC20.1606425702811245No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.1606425702811245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG20.1606425702811245No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG20.1606425702811245No Hit
GCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.1606425702811245Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTA20.1606425702811245Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTATAACACATCTCCGAGCCCACGAGACCAATAGTAGCCGATCTCGTAT20.1606425702811245No Hit
ACCCATGATCTAGTCCCGATCCGACGCACCAAGGTCAAAGATATTCCGAT20.1606425702811245No Hit
TAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTA20.1606425702811245No Hit
GACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCG20.1606425702811245No Hit
CTCCAGGCCCGCGCTAGTTCGAGCAGATAGCGCGGGAACCACGTCAGGGC20.1606425702811245No Hit
GTACTCGCGTGACTGAATCCCCCTTCCCTCGTTAAACCACGGCTCGGGTA20.1606425702811245No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG20.1606425702811245No Hit
GTGTAGGCACGAAGAAATCTAGGGGACCGACGGTCGCCTTCCTCCTTATC20.1606425702811245No Hit
ACATAGGGGTCTATTAGCGCATGTTCGCCGTCTACCGCGCCAGCACGCGA20.1606425702811245No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1606425702811245Clontech SMART CDS Primer II A (96% over 26bp)
CACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGG20.1606425702811245No Hit
TATCAACGCAGAGTACATGGGTACATCGTCGACACCCCCGGCCACGCGGA20.1606425702811245No Hit
GTGCTGATGTGGTCGACGCCCGCGTTGGTGCGCGAGTCGGACAGGAACAC20.1606425702811245No Hit
GGTTTGCGCAGGTCGTATTGCCGGAATGGCGAGCATGGCAGAAACAAATC20.1606425702811245No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG20.1606425702811245No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGGTA20.1606425702811245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCCAGATTGATTAATGTTGGGTTTGCCTTTCTCTTCTGTAAAGGCTTG20.1606425702811245No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCGCCCGTATCCTGCGAAGATCCTTCTTGATGCAGATGATCGTCACG20.1606425702811245No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCAATAGTAGCCGAGCGCGTATG20.1606425702811245No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC20.1606425702811245No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC20.1606425702811245No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)