FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003639270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245
Sequences flagged as poor quality0
Sequence length100
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34827.951807228915666No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA685.461847389558233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT423.3734939759036147Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT221.7670682730923692Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT191.5261044176706828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG181.4457831325301205Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG151.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC141.1244979919678715Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG141.1244979919678715Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC131.0441767068273093Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG90.7228915662650602Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90.7228915662650602Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC80.642570281124498Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC80.642570281124498Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC80.642570281124498Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70.5622489959839357Clontech Universal Primer Mix Long (100% over 23bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60.48192771084337355Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60.48192771084337355Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC60.48192771084337355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50.4016064257028112Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.4016064257028112Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40.321285140562249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT40.321285140562249No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT40.321285140562249No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT40.321285140562249No Hit
GTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACG40.321285140562249No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.321285140562249Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40.321285140562249Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG40.321285140562249Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG30.24096385542168677No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT30.24096385542168677No Hit
AAACCCACCAGGCCAAAACCTGGTGGGAGTAGGCCACGGTTCGCCAGATC30.24096385542168677No Hit
TAACACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAGGCGAGGTTGTGCCGTGACCGTTGTGCCGTGGAACATCTCAGCCG30.24096385542168677No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GGTTATTGCTGACTGCGCAACTATTTCCGACTATACACAAGGTCGTGTTT30.24096385542168677No Hit
GCGTGATTCCGATCGATTCTATCTATACGCCAGTTTCCCGTGTATCTTAT30.24096385542168677No Hit
GTGTGTGGCTATTTTGGTGCATGCCCGGTTGAGTGTGCGGAGGTGATGGT30.24096385542168677No Hit
ACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGAC30.24096385542168677No Hit
AAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAG30.24096385542168677No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCT30.24096385542168677No Hit
TTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAG30.24096385542168677No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC30.24096385542168677No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.24096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.24096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGACGGATGGTGATCCGTGTGCTTTCTGCGCAATGTTGGCTACGAGG20.1606425702811245No Hit
GCCGTGGGTGCCGGCGGGCTGTTGCTGGCGGCTCTTCAGGACCACCTTGA20.1606425702811245No Hit
CTTGGATTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACTAGCCTATCG20.1606425702811245No Hit
GCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCT20.1606425702811245No Hit
GAGTGGATCTGTGAGGTCGATCGCGGCCAGTTGCACCCCTACGAGGGCAA20.1606425702811245No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20.1606425702811245No Hit
ATCCCGTTCCAGGCTGACTCAGGACGCTGTTCATCGTCGCCGCCGCGGCG20.1606425702811245No Hit
GGACATAAGTCTTCTCTCCTGGAATTGCTTGATGCATATATTTAGGCAGA20.1606425702811245No Hit
GAGTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC20.1606425702811245No Hit
TATCTCACCATTGGCTGCTTTCGCAGCGTGACGACTCGTCCCGGCGCGCC20.1606425702811245No Hit
CAAAGAAGTCGACGAAGCGCTGTCCGATCTCCGCTGTTCTCATGCCTAGC20.1606425702811245No Hit
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GCTCGGCCTCGATCATGTGCGCCTATTTCCGCTCTGGCCGTTGTTACAGC20.1606425702811245No Hit
CCTTATAGGTGGGGCTACACACGTCATACAATGGCTGGTACAAAGGGTTG20.1606425702811245No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT20.1606425702811245No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA20.1606425702811245No Hit
CTCCATATCTGGGGTTCGCGAATTGAAACCGAAATGGGCAGGTTATTGCT20.1606425702811245No Hit
ACCTCGTGGTGTTGGTGTCGACGCAGCGTTCCGGTGGACCAGCGTGGTTC20.1606425702811245No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.1606425702811245Clontech Universal Primer Mix Long (96% over 25bp)
GAACGACGCCTTGCGCAGGCTCCCGACAATGACTCCGATGGTCAGATTGC20.1606425702811245No Hit
GTTGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
AACATGATCCTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTC20.1606425702811245No Hit
GCGCCGATGATACCGATGAATTGAGGATATTTCTCAATTCTACCGGAGCT20.1606425702811245No Hit
ACGCAGAGTACATGGGGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA20.1606425702811245No Hit
GTATCAACGCAGAGTACATGGGGGGGAATTCAGATAAAACTAATAGCTCG20.1606425702811245No Hit
ACGCAGAGTACATGGGAGAAAGATCATGTTTATTGGAAGCATCAACCTGC20.1606425702811245No Hit
ACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGC20.1606425702811245No Hit
ATCGAGGTGCGCACGCATGCCGACCTTTGCGTGCTGATGCCCGAAGTCGC20.1606425702811245No Hit
GTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGA20.1606425702811245No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA20.1606425702811245No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT20.1606425702811245No Hit
TGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACAC20.1606425702811245No Hit
GCCCCTTCAACATCGGAGCGCGCCTCGCGCAAAGGGAGGGCCTCATGAGC20.1606425702811245No Hit
CCCTTGTCCAACAGAATCTGGCGGGTTGCTGCTGAATGGGCCGCAAACTC20.1606425702811245No Hit
ACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGA20.1606425702811245No Hit
GCTATCGCTCCTTGTCTACGTGGTATCCCTCACAGCCGATAGCTGAAAAC20.1606425702811245No Hit
GTACATGGGGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTC20.1606425702811245No Hit
GGTCTGGATCTGCCGGTTTCCAAGAGGGATCTGGTGGTGCCGTGGGTAAG20.1606425702811245No Hit
ATGACGACCTAATTGGCGCACAGTACTAGGCATCGTCATCCAATGCGACG20.1606425702811245No Hit
ATCCTGCACAGCGCCACGGCGCAGTCGCTGGTGCTGATGGACGAGATCGG20.1606425702811245No Hit
GGAAAGCGATATGAGTCCAGAAGGCTTCTCCGTCGACACCCATCTCTTGC20.1606425702811245No Hit
ATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGT20.1606425702811245No Hit
TCCATGCCCCACTGTTTGTCAAAGCTCTCCCGGTCCTCCAAGGTGTAGGA20.1606425702811245No Hit
GCGGTGGGGTCGCCCTTCTGCGAGGGCACGTGCTTCAGGATGGTGTCGAA20.1606425702811245No Hit
GAGTCGCGATATGAAGATCAGGATTTAAGATCTCTACATCACTATCGTGT20.1606425702811245No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA20.1606425702811245No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GCCGACGCGCTGTGGGCCGCGGTCGGCACGGTCGAAACGCAGCGCGGCGA20.1606425702811245No Hit
TCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGAT20.1606425702811245No Hit
GTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACC20.1606425702811245No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT20.1606425702811245No Hit
GGCCTGGTGCTGGCGCTGACCGTGCTGGCCGTGAGCTTCCTTTCCTATTC20.1606425702811245No Hit
CGGCGTGGCCTTCGGCCATGGCACGGCCAACGCCTTCGACGAAGCCGCCT20.1606425702811245No Hit
ATCTTCTTCCGTCTTGTTCGCAAGCTCTTGAACCGTGTTAATACCCGCAC20.1606425702811245No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1606425702811245Clontech Universal Primer Mix Long (96% over 26bp)
GCTGTCGACACCGTCACGAATCTTGCCCGGCAAGAACACGATGACGAAGG20.1606425702811245No Hit
GAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGT20.1606425702811245No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20.1606425702811245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGCGCACCAGCCGCACGCCGTATCTGAAGGCGATCATGGACTGCCTGT20.1606425702811245No Hit
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC20.1606425702811245No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)