FastQCFastQC Report
Tue 10 May 2022
EGAF00003639339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196343
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATG128456.542122713822239No Hit
ATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCG105415.368666058886744No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATG86514.4060648966349705No Hit
ATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCG78744.010328863264797No Hit
ACACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTC68123.469438686380467No Hit
ACACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCGTC61123.1129197373983284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49872.539942855105606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41292.102952486210356Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34541.7591663568347227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTCTTC26101.329306366919116Illumina PCR Primer Index 9 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19380.9870481758962631Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19080.9717687923684573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17770.9050488176303714Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTCT15610.7950372562301686No Hit
CACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCGTCT15510.7899441283875666No Hit
CATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCGTCTTC15480.7884161900347861No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13460.6855350076142261Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13230.6738208135762416Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11690.5953866448001711Clontech Universal Primer Mix Long (96% over 26bp)
ACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTCTT10130.5159338504555803RNA PCR Primer, Index 39 (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9730.49556133908517236Clontech SMART CDS Primer II A (96% over 26bp)
TCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTCTTCTG9460.481809893910147RNA PCR Primer, Index 39 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGC9390.4782447044203257No Hit
TCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCGTCTTCTG7660.39013359274331144Illumina PCR Primer Index 3 (95% over 21bp)
ACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCCGTCTT7380.3758728347840259No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7290.3712890197256841Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6710.3417488782385927Clontech Universal Primer Mix Long (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGC6670.33971162710155184No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6620.3371650631802509No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6590.33563712482747027Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6510.3315626225533887Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6160.31373667510428177Clontech Universal Primer Mix Long (100% over 23bp)
TATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCC6160.31373667510428177No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6140.31271804953576143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC6050.3081342344774196Clontech Universal Primer Mix Long (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACACTCCCGCACACATCGCGTATGCC5850.29794797879221563No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5780.2943827893023943Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.28317790804866994No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5530.28164996969588935Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5240.2668798989523436Clontech Universal Primer Mix Long (95% over 21bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4820.2454887620134153Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4650.23683044468099193Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.21187411825224226No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4160.21187411825224226Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4050.2062716776253801Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3910.1991412986457373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3890.1981226730772169No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACACTCCCG3780.19252023245035474No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3770.19201091966609454Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3650.18589916625497216Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3630.18488054068645177Clontech SMART CDS Primer II A (100% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3330.16960115715864585Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA3260.16603596766882447Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2970.15126589692527873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT2670.13598651339747278Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2640.1344585750446922Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2600.1324213239076514Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.12987475998635042No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2460.12529094492800863Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.12223506822244745No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2400.12223506822244745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2380.12121644265392706Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA2190.1115394997529833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA2090.10644637191038132No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT2090.10644637191038132Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2070.10542774634186092No Hit
CTCCGAGCCCACGAGACACTCCCGCACACATCTCGTATGCCGTCTTCTGC2070.10542774634186092Illumina PCR Primer Index 9 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)