FastQCFastQC Report
Tue 10 May 2022
EGAF00003639343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022479
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA612555.990832085548945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG262462.5668986844717594Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG220262.154176271590908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT195521.9122153120015177Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC179751.7579823155292187Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG159741.5622814747295544Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT129891.270343938604118Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA123831.2110762177022707Clontech Universal Primer Mix Long (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATG119441.1681413505803053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT87340.8541984725358662Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77780.7607002197600146Clontech SMART CDS Primer II A (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATGCCG75780.7411399158320122No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT66560.650966914723921Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA64230.6281791606477982Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC63440.6204528405962372Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG62530.6115529023089961Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA58940.5764421567582316Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG57330.5606961120961897Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG57080.5582510741051894Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT54000.5281282060560657Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCGCGTATG51840.507003077813823No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA48250.4718923322630587Clontech Universal Primer Mix Long (95% over 22bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA45250.44255187637105503Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC45110.44118265509609483Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATGCCGTC43480.4252410073947729RNA PCR Primer, Index 26 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA42950.4200575268538523Clontech Universal Primer Mix Long (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCGCGTATGCCG40280.393944521109969No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT39810.3893478496868884Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39420.38553359042092794Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC39230.3836753615477677Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT39110.3825017433120876Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37720.3689073320821259Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG37590.36763591232680576Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC37200.36382165306084524Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA37090.3627458363448051Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA36160.353650295018284Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA33350.3261680679994406Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG32230.31521429779975924Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30900.3022066956876376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCGGGCAATGAACATCGCGTATGCCGTC29390.28743866622199576No Hit
CATCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATGCCGTCTTC29270.28626504798631563RNA PCR Primer, Index 26 (95% over 24bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT28800.28166837656323507Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC28360.2773651096990745Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT27870.27257283523671394Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC27720.27110581244211374Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG27260.2666069425386732Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC25830.2526213252301514Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT25420.24861146292491093Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25220.2466554325321107Clontech Universal Primer Mix Long (95% over 22bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC22750.22249845718102768Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT22140.2165325644829869Clontech SMART CDS Primer II A (100% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20310.1986348863888647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC19250.1882679253070234Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT17510.17125046088966128Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT16100.1574604466204196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14740.1441594399493779Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT14440.1412253943601776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT13930.13623751685853694Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACCGGGCAATGAACATCGCGTATGCCGTCTTC13300.1300760211212162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.12909800592481604No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12770.12489254058029554Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATGCCGTCT12630.12352331930533537RNA PCR Primer, Index 26 (95% over 22bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12460.12186069347145516Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG12230.11961125851973488Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11890.11628600685197447Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACCGGGCAATGAACATCTCGTATGCCGTCTTCTG11590.1133519612627741RNA PCR Primer, Index 19 (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11460.11208054150745395Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.11178713694853391No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.10464762601481302Clontech SMART CDS Primer II A (100% over 23bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10660.10425641993625297Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAA10300.10073556522921254Clontech Universal Primer Mix Long (95% over 21bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10240.10014875611137246Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)